Description of Cylindrospermum solincola sp. nov. from Jammu and Kashmir, India and Further Insights into the Ecological Distribution and Morphological Attributes of Cylindrospermum badium

被引:4
作者
Kumar, Naresh [1 ]
Saraf, Aniket [2 ]
Pal, Sagarika [1 ]
Singh, Prashant [1 ]
机构
[1] Banaras Hindu Univ, Inst Sci, Dept Bot, Varanasi 221005, India
[2] Ramniranjan Jhunjhunwala Coll, Dept Biol Sci, Mumbai 400086, India
来源
DIVERSITY-BASEL | 2023年 / 15卷 / 05期
关键词
cyanobacteria; Cylindrospermum; India; 16S rRNA gene; 16S-23S ITS; polyphasic approach; CYANOBACTERIA; CLASSIFICATION; STRAINS; CHOICE; TREE; TOOL;
D O I
10.3390/d15050592
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
Two cyanobacterial strains KUT1-PS and 18C-PS were collected from the soil surface and vernal pool, respectively, from the Basantgarh village, Udhampur district of the union territory of Jammu and Kashmir, India and characterized by a polyphasic approach. The morphological characterization indicated that both the strains showed typical Cylindrospermum-like morphology and probably belonged to the genus Cylindrospermum. Further, phylogenetic interpretations at the genus level were made using the 16S rRNA gene while the 16S-23S ITS region phylogenetic analysis and secondary structure analysis were conducted to enhance the resolution at the species level. The results from the comparative morphological analysis, the 16S rRNA gene percent similarity and phylogenetic analyses, the 16S-23S ITS percent dissimilarity and the ITS secondary structure analyses provided enough evidence that the strain 18C-PS is a representative of Cylindrospermum badium, providing further insights into its ecological distribution and morphological attributes. Additionally, the strain KUT1-PS was a novel species of the genus Cylindrospermum and is referred to herein as Cylindrospermum solincola sp. nov., in accordance with the International Code of Nomenclature for algae, fungi and plants. This study also discusses the importance of comparing the newly sequenced strains with previously established species before making final taxonomic interpretations.
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