Peptide extraction from silver carp (Hypophthalmichthys molitrix) scales via enzymatic hydrolysis and membrane filtration

被引：6

作者：

Zu, Xiao-Yan ^{[1
,2
]}

Huang, Ya-Qi ^{[1
]}

Zhao, Ya-Jing ^{[1
,3
]}

Xiong, Guang-Quan ^{[1
,2
]}

Liao, Tao ^{[1
,2
]}

Li, Hai-Lan ^{[1
,2
,4
]}

机构：

[1] Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Wuhan, Peoples R China

[2] Minist Agr & Rural Affairs, Key Lab Agr Prod Cold Chain Logist, Wuhan, Peoples R China

[3] Lanzhou Univ Technol, Coll Petrochem Technol, Lanzhou, Peoples R China

[4] Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, 5 Nanhu Ave, Wuhan 430064, Hubei, Peoples R China

来源：

ITALIAN JOURNAL OF FOOD SCIENCE | 2023年 / 35卷 / 02期

关键词：

membrane filtration; peptide extraction; response surface methodology; silver carp scales; NANOFILTRATION MEMBRANES; AMINO-ACIDS; COLLAGEN; SKIN;

D O I：

10.15586/ijfs.v35i2.2248

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this work, peptides were extracted from Silver carp (SC) scales via protease hydrolysis and separated using two membranes (M1 and M2). The results revealed that the water: SC scale ratios of 50.6 mL/g, alkaline protease 1 (AP1) dose of 2313.6 U/mL, and pH of 8.14 were the optimal hydrolysis conditions, and the peptide yield reached 88.77 +/- 0.32%. The optimal conditions of peptide separation were clarified: the operating pressure of the M1 (M2) was 0.25 (0.4) MPa, the liquid temperature was 30 degrees C, and the operation time was 65 min. In this case, the per-meability of the M1 (M2) reached 91.73 +/- 96% (79.83 +/- 7.23%), and the average molecular weight of the peptides was 758 Da (576 Da). Compared with M1 peptides, M2 peptides contained more acidic and aromatic amino acids exhibiting free-radical scavenging and tyrosinase inhibition properties. It might provide a way to utilize SC scales as a promising material to produce bioactive peptides.

引用

页码：44 / 53

页数：10