Axonal plasticity in response to active forces generated through magnetic nano-pulling

被引：21

作者：

Falconieri, Alessandro ^{[1
]}

De Vincentiis, Sara ^{[1
,2
]}

Cappello, Valentina ^{[3
]}

Convertino, Domenica ^{[4
]}

Das, Ravi ^{[2
]}

Ghignoli, Samuele ^{[1
]}

Figoli, Sofia ^{[1
]}

Luin, Stefano ^{[5
]}

Catala-Castro, Frederic ^{[2
]}

Marchetti, Laura ^{[4
,6
]}

Borello, Ugo ^{[1
]}

Krieg, Michael ^{[2
]}

Raffa, Vittoria ^{[1
]}

机构：

[1] Univ Pisa, Dept Biol, I-56127 Pisa, Italy

[2] ICFO, Barcelona Inst Sci & Technol, Inst Ciencies Foton, Castelldefels 08860, Spain

[3] Ist Italiano Tecnol, Ctr Mat Interfaces, I-56025 Pontedera, Italy

[4] Ist Italiano Tecnol, Ctr Nanotechnol Innovat NEST, I-56127 Pisa, Italy

[5] Scuola Normale Super Pisa, Natl Enterprise Nanosci & NanoTechnol NEST Lab, I-56127 Pisa, Italy

[6] Univ Pisa, Dept Pharm, I-56126 Pisa, Italy

来源：

CELL REPORTS | 2023年 / 42卷 / 01期

基金：

欧洲研究理事会;

关键词：

C; ELEGANS; MICROTUBULE DYNAMICS; MECHANICAL CONTROL; CORTICAL-NEURONS; TAU-PROTEIN; IN-VIVO; GROWTH; TRANSPORT; PHOSPHORYLATION; INHIBITION;

D O I：

10.1016/j.celrep.2022.111912

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mechanical force is crucial in guiding axon outgrowth before and after synapse formation. This process is referred to as "stretch growth."However, how neurons transduce mechanical input into signaling pathways remains poorly understood. Another open question is how stretch growth is coupled in time with the interca-lated addition of new mass along the entire axon. Here, we demonstrate that active mechanical force gener-ated by magnetic nano-pulling induces remodeling of the axonal cytoskeleton. Specifically, the increase in the axonal density of microtubules induced by nano-pulling leads to an accumulation of organelles and signaling vesicles, which, in turn, promotes local translation by increasing the probability of assembly of the "translation factories."Modulation of axonal transport and local translation sustains enhanced axon outgrowth and synapse maturation.

引用

页数：27

共 111 条

[1] Tension directly stabilizes reconstituted kinetochore-microtubule attachments [J].