Assessing the impact of an antigen-specific antibody response on atherosclerosis development in mice

被引：0
作者：
Del Monte-Monge, Alberto [1 ]
Martos-Folgado, Inmaculada [1 ]
Rodriguez-Ronchel, Ana [1 ]
Ramiro, Almudena R. [1 ]
机构：
[1] Ctr Nacl Invest Cardiovasc CNIC, Lymphocyte Biol Lab B, Madrid, Spain
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 02期
关键词：
LOW-DENSITY-LIPOPROTEIN; E-DEFICIENT MICE; HYPERCHOLESTEROLEMIA; IMMUNIZATION; INDUCTION; CELLS;
D O I：
10.1016/j.xpro.2023.102274
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived anti-bodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1
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页数：13
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