Self-Renewable Tag for Photostable Fluorescence Imaging of Proteins

被引：11

作者：

Du, Zhichao ^{[1
]}

Wang, Wenjing ^{[1
]}

Luo, Siyuan ^{[1
]}

Zhang, Lingjie ^{[1
]}

Yuan, Shuowei ^{[1
]}

Hei, Yongzhen ^{[2
]}

Bao, Zhangbin ^{[3
]}

Chen, Chunlai ^{[2
]}

Lin, Yi ^{[3
]}

Chu, Ling ^{[4
]}

机构：

[1] Tsinghua Univ, Sch Pharmaceut Sci, Beijing 100084, Peoples R China

[2] Tsinghua Univ, Beijing Adv Innovat Ctr Struct, Beijing Frontier Res Ctr Biol Struct, Sch Life Sci, Beijing 100084, Peoples R China

[3] Tsinghua Peking Joint Ctr Life Sci, IDG McGovern Inst Brain Res, Beijing 100084, Peoples R China

[4] Tsinghua Univ, Sch Pharmaceut Sci, MOE Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2023年 / 145卷 / 34期

关键词：

LIVE-CELL; GENERAL-METHOD; SUPERRESOLUTION MICROSCOPY; FLUOROGENIC PROBES; FLUOROPHORES; NANOSCOPY;

D O I：

10.1021/jacs.3c06102

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the development of a self-renewable tag (srTAG)for proteinfluorescence imaging. srTAG leverages the "on-protein"fluorophore equilibrium between the fluorescent zwitterion and non-fluorescentspirocyclic form and the reversible fluorescence labeling to enableself-recovery of fluorescence after photobleaching. This small-sizedsrTAG allows 2-6 times longer imaging duration compared toother commonly used self-labeling tags and is compatible with fluorophoreswith different spectral properties. This study provides a new strategyfor fine tuning of self-labeling tags.

引用

页码：18968 / 18976

页数：9

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