Pyrene-Labeled and Quaternized Chitosan: Synthesis, Characterization, and Its Potential Application for Fluorescently Trackable Nucleic Acid Delivery into Cells

被引：1

作者：

Pratumyot, Kornkanya ^{[3
,4
]}

Yuntasiri, Pongsakorn ^{[3
,4
]}

Khunsuk, Phim-on ^{[5
]}

Phuangkaew, Tinnakorn ^{[1
]}

Sittplangkoon, Chutamath ^{[6
]}

Pattarakankul, Thitiporn ^{[7
,8
]}

Palaga, Tanapat ^{[2
,7
,8
]}

Kiatkamjornwong, Suda ^{[9
,10
]}

Hoven, Voravee P. ^{[1
,2
]}

机构：

[1] Chulalongkorn Univ, Fac Sci, Dept Chem, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Ctr Excellence Mat & Biointerfaces, Bangkok 10330, Thailand

[3] King Mongkuts Univ Technol Thonburi KMUTT, Fac Sci, Dept Chem, Organ Synth Electro Chem & Nat Prod Res Unit, Bangkok 10140, Thailand

[4] King Mongkuts Univ Technol Thonburi KMUTT, Fac Sci, Dept Chem, Supramol Chem Res Unit, Bangkok 10140, Thailand

[5] Chulalongkorn Univ, Fac Sci, Program Petro Chem & Polymer Sci, Bangkok 10330, Thailand

[6] Chulalongkorn Univ, Fac Sci, Grad Program Biotechnol, Bangkok 10330, Thailand

[7] Chulalongkorn Univ, Fac Sci, Dept Microbiol, Bangkok 10330, Thailand

[8] Chulalongkorn Univ, Ctr Excellence Mat & Biointerfaces, Bangkok 10330, Thailand

[9] Off Royal Soc, Acad Sci, FRST, Bangkok 10300, Thailand

[10] Chulalong korn Univ, Off Res Affairs, Bangkok 10330, Thailand

来源：

BIOMACROMOLECULES | 2023年 / 24卷 / 09期

关键词：

GENE DELIVERY; IN-VITRO; SIRNA DELIVERY; TRANSFECTION EFFICIENCY; INTRACELLULAR DRUG; AMINO-GROUPS; NANOPARTICLES; SPERMINE; MECHANISM; PRECURSOR;

D O I：

10.1021/acs.biomac.3c00301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A chitosan derivative (Pyr-CS-HTAP)having pyrene (Pyr)and N-[(2-hydroxyl-3-trimethylammonium)] propyl (HTAP)unitsconjugated at C6 and C2 positions, respectively, was synthesized andcharacterized. Dynamic light scattering and scanning electron microscopyrevealed that Pyr-CS-HTAP self-assembled into spherical nanoparticleswith a hydrodynamic diameter of 211 & PLUSMN; 5 nm and a & zeta;-potentialof +49 mV. The successful binding of Pyr-CS-HTAP with nucleic acidwas ascertained by fluorescence resonance energy-transfer analysisand gel electrophoresis. Pyr-CS-HTAP facilitated the cellular uptakeof nucleic acid up to 99%. Co-localization analysis using fluorescencemicroscopy revealed the endosomal escape of the Pyr-CS-HTAP/nucleicacid complexes and the successful release of the nucleic acid cargoesfrom the polyplexes into the nucleus. It is strongly believed thatPyr-CS-HTAP can potentially be developed into a fluorescently trackablegene delivery system in the future.

引用

页码：4005 / 4018

页数：14

共 7 条

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