Assessing Onchocerca volvulus Intensity of Infection and Genetic Diversity Using Mitochondrial Genome Sequencing of Single Microfilariae Obtained before and after Ivermectin Treatment

被引：4

作者：

Hedtke, Shannon M. ^{[1
]}

Choi, Young-Jun ^{[2
,3
]}

Kode, Anusha ^{[1
]}

Chalasani, Gowtam C. ^{[1
]}

Sirwani, Neha ^{[1
]}

Jada, Stephen R. ^{[4
]}

Hotterbeekx, An ^{[5
]}

Mandro, Michel ^{[6
]}

Fodjo, Joseph N. Siewe N. ^{[5
]}

Amambo, Glory Ngongeh ^{[7
]}

Abong, Raphael A. ^{[7
,8
]}

Wanji, Samuel ^{[7
,8
]}

Kuesel, Annette C. ^{[9
]}

Colebunders, Robert ^{[5
]}

Mitreva, Makedonka ^{[2
,3
]}

Grant, Warwick N. ^{[1
]}

机构：

[1] La Trobe Univ, Sch Agr Biomed & Environm, Dept Environm & Genet, Bundoora, Vic 3086, Australia

[2] Washington Univ St Louis, Dept Med, St Louis, MO 63108 USA

[3] McDonnell Genome Inst, St Louis, MO 63108 USA

[4] Amref South Sudan, POB 30125, Juba, South Sudan

[5] Univ Antwerp, Global Hlth Inst, Doornstraat 331, B-2610 Antwerp, Belgium

[6] Minist Hlth, Prov Hlth Div Ituri, POB 57, Bunia, Rep Congo

[7] Univ Buea, Fac Sci, Dept Microbiol & Parasitol, Paras & Vectors Res Unit, POB 63, Buea, Cameroon

[8] Res Fdn Trop Dis & Environm REFOTDE, POB 474, Buea, Cameroon

[9] WHO, World Hlth Org Special Programme Res & Training T, UNICEF, UNDP,World Bank, CH-1202 Geneva, Switzerland

来源：

PATHOGENS | 2023年 / 12卷 / 07期

基金：

欧洲研究理事会;

关键词：

onchocerciasis; population genetics; microfilariae; epidemiology; macrofilariae; elimination; monitoring; drug studies; VOLTA RIVER BASIN; CONTROL PROGRAM; SUBOPTIMAL RESPONSES; MULTIPLE TREATMENTS; SPECIES RICHNESS; ADULT PARASITES; COMOE VALLEY; ENDEMIC FOCI; REINVASION; RECRUDESCENCE;

D O I：

10.3390/pathogens12070971

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Onchocerciasis is a neglected tropical disease targeted for elimination using ivermectin mass administration. Ivermectin kills the microfilariae and temporarily arrests microfilariae production by the macrofilariae. We genotyped 436 microfilariae from 10 people each in Ituri, Democratic Republic of the Congo (DRC), and Maridi County, South Sudan, collected before and 4-5 months after ivermectin treatment. Population genetic analyses identified 52 and 103 mitochondrial DNA haplotypes among the microfilariae from DRC and South Sudan, respectively, with few haplotypes shared between people. The percentage of genotype-based correct assignment to person within DRC was similar to 88% and within South Sudan similar to 64%. Rarefaction and extrapolation analysis showed that the genetic diversity in DRC, and even more so in South Sudan, was captured incompletely. The results indicate that the per-person adult worm burden is likely higher in South Sudan than DRC. Analyses of haplotype data from a subsample (n = 4) did not discriminate genetically between pre- and post-treatment microfilariae, confirming that post-treatment microfilariae are not the result of new infections. With appropriate sampling, mitochondrial haplotype analysis could help monitor changes in the number of macrofilariae in a population as a result of treatment, identify cases of potential treatment failure, and detect new infections as an indicator of continuing transmission.

引用

页数：20