ISOLATION OF A NOVEL ANTIMICROBIAL POLYPEPTIDE FROM AN Aspergillus niger ISOLATE

被引：0

作者：

Ustun, Ayse ^{[1
,2
]}

Yazici, Aysenur ^{[1
,2
]}

Ortucu, Serkan ^{[1
,2
]}

机构：

[1] Erzurum Tech Univ, Fac Sci, Dept Mol Biol & Genet, Erzurum, Turkiye

[2] Erzurum Tech Univ, High Technol Res & Applicat Ctr, Erzurum, Turkiye

来源：

TRAKYA UNIVERSITY JOURNAL OF NATURAL SCIENCES | 2023年 / 24卷 / 02期

关键词：

Antimicrobial polypeptide; Aspergillus niger; Gel overlay assay; Staphylococcus aureus; PEPTIDE; ANTIBIOTICS;

D O I：

10.23902/trkjnat.1247186

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In this study the extracellular proteins from the isolate LC3 belonging to Aspergillus were purified for new antimicrobial polypeptide (AMP) discovery and then tested for antimicrobial activity against Staphylococcus aureus (ATCC 25923) and Methicillinresistant S. aureus (MRSA). Antimicrobial activity was determined by the trypsin/proteinase K assay, which was polypeptide-based, and it was observed that this protein was a protein of about 11 kDa by gel overlay assay. The minimum inhibitory concentration of purified AMP molecule against S. aureus ATCC 25923 and MRSA was 8 mu g/ml and 32 mu g/ml, respectively and the AMP molecule was confirmed. ITS sequence analysis showed that isolate LC3 was identified as Aspergillus niger, using the Bioedit sequence assembly program. The sequence was deposited with the GenBank database with accession number MK332597. The results indicate that the purified AMP molecule has the potential to be used in infections caused by S. aureus.

引用

页码：41 / 48

页数：8