Cryo-EM structures of actin binding proteins as tool for drug discovery

被引：2
|
作者：
Dahlstroem, Christian [1 ]
Paraschiakos, Themistoklis [1 ]
Sun, Han [2 ,3 ]
Windhorst, Sabine [1 ]
机构：
[1] Univ Med Ctr Hamburg Eppendorf, Dept Biochem & Signal Transduct, Martinistr 52, D-20246 Hamburg, Germany
[2] Leipniz Forsch inst Moekure Pharmakol, Struct Chem & Comp Biophys Grp, Robert-Rossle-Str 10, D-13125 Berlin, Germany
[3] Tech Univ Berlin, Inst Chem, D-10623 Berlin, Germany
来源：
BIOCHEMICAL PHARMACOLOGY | 2023年 / 214卷
关键词：
Actin; Cytoskeleton; Drug design; Cryo-EM; Cancer; Metastasis; L-PLASTIN; DEPOLYMERIZING FACTOR; FILAMENT TURNOVER; KABIRAMIDE-C; COFILIN; EXPRESSION; ADF/COFILIN; DYNAMICS; SLINGSHOT; PROMOTES;
D O I：
10.1016/j.bcp.2023.115680
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Cellular actin dynamic is controlled by a plethora of actin binding proteins (ABPs), including actin nucleating, bundling, cross-linking, capping, and severing proteins. In this review, regulation of actin dynamics by ABPs will be introduced, and the role of the F-actin severing protein cofilin-1 and the F-actin bundling protein L-plastin in actin dynamics discussed in more detail. Since up-regulation of these proteins in different kinds of cancers is associated with malignant progression of cancer cells, we suggest the cryogenic electron microscopy (Cryo-EM) structure of F- actin with the respective ABP as template for in silico drug design to specifically disrupt the interaction of these ABPs with F-actin.
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收藏
页数：11
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