MicroRNA-146a-5p induces cell cycle arrest and enhances apoptosis in gastric cancer via targeting CDC14A

被引：6

作者：

Jiang, Piao ^{[1
,2
]}

Liang, Bin ^{[1
]}

Zhang, Zhen ^{[3
]}

Fan, Bing ^{[4
]}

Zeng, Lin ^{[1
]}

Zhou, Zhiyong ^{[1
]}

Mao, Zhifang ^{[1
]}

Lin, Qing ^{[5
]}

Yao, Weirong ^{[1
]}

Shen, Qinglin ^{[1
,3
]}

机构：

[1] Nanchang Med Coll, Jiangxi Prov Peoples Hosp, Affiliated Hosp 1, Dept Oncol, Nanchang, Peoples R China

[2] Nanchang Univ, Clin Med Coll 1, Nanchang, Peoples R China

[3] Nanchang Med Coll, Affiliated Hosp 1, Jiangxi Prov Peoples Hosp, Inst Clin Med, Nanchang, Peoples R China

[4] Nanchang Med Coll, Jiangxi Prov Peoples Hosp, Affiliated Hosp 1, Dept Radiol, Nanchang, Peoples R China

[5] Nanchang Med Coll, Jiangxi Prov Peoples Hosp, Affiliated Hosp 1, Dept Thorac Surg, Nanchang, Peoples R China

来源：

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY | 2023年 / 11卷

关键词：

microRNA-146a-5p; gastric cancer; CDC14A; cell cycle; apoptosis; COLORECTAL-CANCER; PROLIFERATION; MIR-193A-5P; MIR-146A-5P; EXPRESSION;

D O I：

10.3389/fcell.2023.1181628

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective: The present study was designed to investigate the expression of miRNA-146a-5p in gastric cancer (GC) tissues and the paired nonmalignant counterparts, to explore the influences of miRNA-146a-5p on the cell biological behavior of MKN-28 cells (highly metastatic human gastric cancer cells), and to identify the function of abnormal expression of its target gene cell division cycle 14 homolog A (CDC14A) in GC.Methods: We detected the expression of miRNA-146a-5p in formalin-fixed and paraffin-embedded (FFPE) GC tissues through microarray and quantitative real-time polymerase chain reaction (qRT-PCR). Then, we employed cell counting kit-8 (CCK-8) assays, cell cycle assays, and apoptosis analysis to uncover the latent function of miRNA-146a-5p in MKN-28 human GC cells. We also validated the target of miRNA-146a-5p via luciferase reporter assays.Results: miRNA-146a-5p levels were examined in the majority of primary GC tissues and several GC cell lines. As a result, miRNA-146a-5p levels were significantly declined in the GC tissues and cells. In addition, miRNA-146a-5p demonstrated a straight act on its 3 & PRIME;-untranslated region (3 & PRIME;-UTR) of CDC14A mRNA, accordingly decreasing the contents of CDC14A mRNA as well as its protein expression. An inverse correlation between CDC14A and miRNA-146a-5p was observed.Conclusion: The data suggest miRNA-146a-5p may contribute to inducing cell cycle arrest as well as prompting GC cell apoptosis via directly targeting CDC14A. Therefore, miRNA-146a-5p may be a potential indicator of the occurrence and development of GC.

引用

页数：9