A Novel DNBS-based Fluorescent Probe for the Detection of H2S in Cells and on Test Strips

被引：3

作者：

Wang, Wenxiang ^{[1
]}

Gan, Yudie ^{[1
]}

Jiang, Huaqin ^{[1
]}

Fang, Min ^{[1
,2
]}

Wu, Zhenyu ^{[1
]}

Zhu, Weiju ^{[1
,3
]}

Li, Cun ^{[4
]}

机构：

[1] Anhui Univ, Sch Chem & Chem Engn, Hefei 230601, Peoples R China

[2] Anhui Univ, Anhui Prov Key Lab Environm Friendly Polymer Mat, Hefei 230601, Peoples R China

[3] Anhui Univ, Anhui Prov Key Lab Chem Inorgan, Organ Hybrid Functionalized Mat, Hefei 230601, Peoples R China

[4] Anhui Univ, Sch Mat Sci & Engn, Hefei 230601, Peoples R China

来源：

JOURNAL OF FLUORESCENCE | 2024年 / 35卷 / 4期

关键词：

Hydrogen sulfide; Fluorescent probe; Fluorescence imaging; Test strips; HYDROGEN-SULFIDE; LIVING CELLS; CYSTEINE;

D O I：

10.1007/s10895-024-03660-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydrogen sulfide (H2S) plays a key role in the physiology and pathology of organisms, and H2S in the environment is easily absorbed and harmful to health. It is of great significance to develop a probe with good selectivity, high sensitivity and good stability that can detect hydrogen sulfide inside and outside organisms. In this work, we designed a novel "turn-on" fluorescent probe CIM-SDB for the detection of H2S. The probe CIM-SDB used indene-carbazole as the fluorophore and 2,4-dinitrobenzenesulfonyl as the recognition site. The probe CIM-SDB exhibited high selectivity and sensitivity to H2S (detection limit as low as 123 nM). Moreover, the probe CIM-SDB was successfully applied to the detection of intracellular exogenous and endogenous H2S, and the test strips prepared by the probe CIM-SDB could realize the convenient and rapid detection of H2S.

引用

页码：2109 / 2120

页数：12

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