Non-mass spectrometric targeted single-cell metabolomics

被引：3

作者：

Cheng, Hanjun ^{[1
]}

Tang, Yin ^{[1
]}

Li, Zhonghan ^{[2
]}

Guo, Zhili ^{[2
]}

Heath, James R. ^{[1
]}

Xue, Min ^{[2
]}

Wei, Wei ^{[1
]}

机构：

[1] Inst Syst Biol, Seattle, WA 98109 USA

[2] Univ Calif Riverside, Dept Chem, Riverside, CA 92521 USA

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2023年 / 168卷

基金：

美国国家卫生研究院;

关键词：

Single -cell metabolism; Surface chemistry; Microfluidic chip; Fluorescent metabolic tracer; Raman microscopy; Single -cell amperometry; VESICULAR EXOCYTOSIS; SIGNALING DYNAMICS; DRUG DISCOVERY; GLUCOSE-UPTAKE; BREAST-CANCER; RNA-SEQ; ACID; TRANSMITTERS; RELEASE; STRESS;

D O I：

10.1016/j.trac.2023.117300

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Metabolic assays serve as pivotal tools in biomedical research, offering keen insights into cellular physiological and pathological states. While mass spectrometry (MS)-based metabolomics remains the gold standard for comprehensive, multiplexed analyses of cellular metabolites, innovative technologies are now emerging for the targeted, quantitative scrutiny of metabolites and metabolic pathways at the single-cell level. In this review, we elucidate an array of these advanced methodologies, spanning synthetic and surface chemistry techniques, imaging-based methods, and electrochemical approaches. We summarize the rationale, design principles, and practical applications for each method, and underscore the synergistic benefits of integrating single-cell metabolomics (scMet) with other single-cell omics technologies. Concluding, we identify prevailing challenges in the targeted scMet arena and offer a forward-looking commentary on future avenues and opportunities in this rapidly evolving field.

引用

页数：14

共 102 条

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