Detection of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita, Colombia

被引：0

作者：

Cruz-Estupinan, Sharon E. ^{[1
]}

Bulla-Castaneda, Diana M. ^{[1
]}

Lancheros-Buitrago, Deisy J. ^{[1
]}

Garcia-Corredor, Diego J. ^{[1
]}

Giraldo Forero, Julio C. ^{[2
,3
]}

Pulido-Medellin, Martin O. ^{[1
]}

机构：

[1] Univ Pedagog & Tecnol Colombia, Grp Invest Med Vet & Zootecnia GIDIMEVETZ, Tunja, Colombia

[2] Univ Mil Nueva Granada Bogota, Fac Med & Ciencias Salud, Grp Invest Ecoepidemiol & Salud Colect, Bogota, Colombia

[3] Univ INCCA Colombia, Fac Ingn Adm & Ciencias Basicas, Grp Invest Parasitol & Microbiol Trop GIPAMT, Programa Biol, Bogota, Colombia

来源：

REVISTA DE INVESTIGACIONES VETERINARIAS DEL PERU | 2023年 / 34卷 / 05期

关键词：

Fasciola hepatica; sheep; goats; indirect ELISA; seroprevalence; FECAL EXAMINATION; PREVALENCE; EPIDEMIOLOGY; DIAGNOSIS; RUMINANTS; AREA;

D O I：

10.15381/rivep.v34i5.26378

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The aim of this study was to determine the seroprevalence of Fasciola hepatica by indirect ELISA in sheep and goats from Boavita ( Boyaca, Colombia). A descriptive cross-sectional study with simple random sampling was carried out. A total of 297 blood samples from sheep and 337 from goats of different breeds and age groups were collected. The sera were analyzed by the indirect ELISA technique with the commercial ELISA kit BIO K 211 - Monoscreen AbELISA F. hepatica. Additionally, an epidemiological survey was carried out. The seroprevalence of F. hepatica was 67.34% (200/297) in sheep and 59.94% (202/337) in goats. Likewise, seroprevalence was higher in males (sheep: 77.78%, 21/27; goats: 63.89%, 23/36), in sheep older than 3 years (83.33%, 50/60) and in goats less than 1 year of age. (69.77%, 60/86), as well as in Creole breed (sheep: 69.84%; goats: 61.79%). Sheep over 3 years of age were determined as a risk factor, while in goats the Creole breed variables and extensive grazing were identified as risk factors.

引用

页数：10

共 50 条

[31] Temporal genetic variation of Fasciola hepatica from sheep in Galicia (NW Spain) [J].

Vazquez-Prieto, Severo ;

Vitas, Roman ;

Ubeira, Florencio M. ;

Paniagua, Esperanza .

VETERINARY PARASITOLOGY, 2015, 209 (3-4) :268-272

[32] Screening anthelmintic resistance to triclabendazole in Fasciola hepatica isolated from sheep by means of an egg hatch assay [J].

Robles-Perez, David ;

Martinez-Perez, Jose Manuel ;

Rojo-Vazquez, Francisco Antonio ;

Martinez-Valladares, Maria .

BMC VETERINARY RESEARCH, 2015, 11

[33] Milk Indirect-ELISA and Milk Ring Test for Screening of Brucellosis in Buffaloes, Goats and Bulk Tank Milk Samples Collected from Two Districts of Punjab, Pakistan [J].

Khan, Tayyaba Israr ;

Ehtisham-ul-Haque, Syed ;

Waheed, Usman ;

Khan, Iahtasham ;

Younus, Muhammad ;

Ali, Shahzad .

PAKISTAN VETERINARY JOURNAL, 2018, 38 (01) :105-108

[34] MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F-gigantica [J].

Adela Valero, M. ;

Ubeira, Florencio M. ;

Khoubbane, Messaoud ;

Artigas, Patricio ;

Muino, Laura ;

Mezo, Mercedes ;

Perez-Crespo, Ignacio ;

Victoria Periago, M. ;

Mas-Coma, Santiago .

VETERINARY PARASITOLOGY, 2009, 159 (01) :77-81

[35] Diagnosis of sheep fasciolosis caused by Fasciola hepatica using cathepsin L enzyme-linked immunosorbent assays (ELISA) [J].

Corrales, Jesus Lopez ;

Cwiklinski, Krystyna ;

Verissimo, Carolina De Marco ;

Dorey, Amber ;

Lalor, Richard ;

Jewhurst, Heather ;

McEvoy, Amanda ;

Diskin, Michael ;

Duffy, Catherine ;

Cosby, S. Louise ;

Keane, Orla M. ;

Dalton, John Pius .

VETERINARY PARASITOLOGY, 2021, 298

[36] Seroepidemiological study of Caseous lymphadenitis in sheep from the Northeast region of Brazil using an indirect ELISA [J].

Alves, Jose Romero Alexandre ;

de Farias, Areano Etherio Moreira ;

dos Anjos, Denize Monteiro ;

Lima, Ana Milena Cezar ;

Faccioli-Martins, Patricia Yoshida ;

de Souza, Carlos Jose Hoff ;

Pinheiro, Raymundo Rizaldo ;

Alves, Francisco Selmo Fernandes ;

de Azevedo, Sergio Santos ;

Alves, Clebert Jose .

TROPICAL ANIMAL HEALTH AND PRODUCTION, 2020, 52 (04) :1945-1952

[37] Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test [J].

Salimi-Bejestani, MR ;

McGarry, JW ;

Felstead, S ;

Ortiz, P ;

Akca, A ;

Williams, DJL .

RESEARCH IN VETERINARY SCIENCE, 2005, 78 (02) :177-181

[38] Performance characteristics of an enzyme-linked immunosorbent assay for the detection of liver fluke (Fasciola hepatica) infection in sheep and cattle [J].

Reichel, MP .

VETERINARY PARASITOLOGY, 2002, 107 (1-2) :65-72

[39] Comparative assessment of faecal diagnostics for detection and predictive modelling of endemic Fasciola hepatica infection in sheep and cattle on Australian farms [J].

George, S. D. ;

George, A. J. ;

Rolfe, P. F. ;

Emery, D. L. .

VETERINARY PARASITOLOGY, 2019, 276

[40] Prevalence of Fasciola hepatica infection in slaughtered sheep from Northwest Tunisia and its risk factors: Association with gastrointestinal helminths infection and anaemia [J].

Hammami, Ines ;

Amdouni, Yosra ;

Romdhane, Rihab ;

Sassi, Limam ;

Farhat, Nadia ;

Rekik, Mourad ;

Gharbi, Mohamed .

VETERINARY MEDICINE AND SCIENCE, 2024, 10 (05)

← 1 2 3 4 5 →