Oxidative Stress and DNA Damage in Pagrus major by the Dinoflagellate Karenia mikimotoi

被引:3
|
作者
Shin, Yun Kyung [1 ]
Seo, Do Yeon [2 ,3 ]
Eom, Hye-Jin [2 ]
Park, Mira [4 ]
Lee, Minji [5 ]
Choi, Young-Eun [2 ,6 ]
Han, Young-Seok [7 ]
Rhee, Jae-Sung [2 ,4 ,8 ]
Kim, Youn-Jung [2 ,4 ,8 ]
机构
[1] Natl Inst Fisheries Sci, Busan 46083, South Korea
[2] Incheon Natl Univ, Dept Marine Sci, Coll Nat Sci, Incheon 22012, South Korea
[3] Natl Inst Environm Res, Environm Hlth Res Dept, Risk Assessment Div, Incheon 22689, South Korea
[4] Incheon Natl Univ, Res Inst Basic Sci, Incheon 22012, South Korea
[5] South Sea Fisheries Res Inst, Natl Inst Fisheries Sci NIFS, Yeosu 59780, South Korea
[6] Eco Sustainable Solut Ctr Korea Conform Labs, Dept Neurol, Incheon 40684, South Korea
[7] Neo Environm Business Co, Bucheon 14523, South Korea
[8] Yellow Sea Res Inst, Incheon 22012, South Korea
基金
新加坡国家研究基金会;
关键词
Karenia mikimotoi; Pagrus major; oxidative stress; DNA damage; RED-TIDE DINOFLAGELLATE; HARMFUL ALGAL BLOOMS; MOLECULAR ECOLOGICAL RESPONSES; ANTIOXIDANT RESPONSES; GYRODINIUM-AUREOLUM; LIPID-PEROXIDATION; HYDROGEN-PEROXIDE; COASTAL WATERS; OXYGEN LEVELS; SEA;
D O I
10.3390/toxins15100620
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Karenia mikimotoi is a common species of red tide dinoflagellate that causes the mass mortality of marine fauna in coastal waters of Republic of Korea. Despite continuous studies on the ecophysiology and toxicity of K. mikimotoi, the underlying molecular mechanisms remain poorly understood. Red sea bream, Pagrus major, is a high-value aquaculture fish species, and the coastal aquaculture industry of red sea bream has been increasingly affected by red tides. To investigate the potential oxidative effects of K. mikimotoi on P. major and the molecular mechanisms involved, we exposed the fish to varying concentrations of K. mikimotoi and evaluated its toxicity. Our results showed that exposure to K. mikimotoi led to an accumulation of reactive oxygen species (ROS) and oxidative DNA damage in the gill tissue of P. major. Furthermore, we found that K. mikimotoi induced the activation of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione peroxidase, and glutathione reductase, in the gill tissue of P. major, with a significant increase in activity at concentrations above 5000 cells/mL. However, the activity of glutathione S-transferase did not significantly increase at the equivalent concentration. Our study confirms that oxidative stress and DNA damage is induced by acute exposure to K. mikimotoi, as it produces ROS and hypoxic conditions in P. major. In addition, it was confirmed that gill and blood samples can be used as biomarkers to detect the degree of oxidative stress in fish. These findings have important implications for the aquaculture of red sea bream, particularly in the face of red tide disasters.
引用
收藏
页数:16
相关论文
共 50 条
  • [21] Inhibition to crucial enzymes in the lethal effects of the dinoflagellate Karenia mikimotoi on the rotifer Brachionus plicatilis
    Li, Xiao-dong
    Yan, Tian
    Zhang, Qing-chun
    Yu, Ren-cheng
    Zhou, Ming-jiang
    MARINE ENVIRONMENTAL RESEARCH, 2020, 157 (157)
  • [22] Cell density-dependent suppression on the development and photosynthetic activities of Sargassum fusiformis embryos by dinoflagellate Karenia mikimotoi
    Shang, Tiange
    Lin, Lidong
    Chen, Binbin
    Wang, Min
    Qin, Wenli
    Dai, Chuanjun
    Yu, Hengguo
    Li, Jun
    Thring, Ronald W.
    Ma, Zengling
    Zhao, Min
    HARMFUL ALGAE, 2020, 96
  • [23] Efficient modulation of cellular phosphorus components in response to phosphorus deficiency in the dinoflagellate Karenia mikimotoi
    Huang, Xue-Ling
    Zhuang, Yan-Qing
    Xiong, Yue-Yue
    Li, Da-Wei
    Ou, Lin-Jian
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2023,
  • [24] Extremely high level of reactive oxygen species (ROS) production in a newly isolated strain of the dinoflagellate Karenia mikimotoi
    Kim, Daekyung
    Li Wencheng
    Matsuyama, Yukihiko
    Cho, Kichul
    Yamasaki, Yasuhiro
    Takeshita, Satoshi
    Yamaguchi, Kenichi
    Oda, Tatsuya
    EUROPEAN JOURNAL OF PHYCOLOGY, 2019, 54 (04) : 632 - 640
  • [25] Size-dependent oxidative stress effect of nano/micro-scaled polystyrene on Karenia mikimotoi
    Zhao, Ting
    Tan, Liju
    Zhu, Xiaolin
    Huang, Wenqiu
    Wang, Jiangtao
    MARINE POLLUTION BULLETIN, 2020, 154
  • [26] Production of superoxide anion and hydrogen peroxide by the red tide dinoflagellate Karenia mikimotoi
    Yamasaki, Y
    Kim, DI
    Matsuyama, Y
    Oda, T
    Honjo, T
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2004, 97 (03) : 212 - 215
  • [27] Proteomic insights of interaction between ichthyotoxic dinoflagellate Karenia mikimotoi and algicidal bacteria Maribacter dokdonensis
    Lee, Thomas Chun-Hung
    Lam, Winnie
    Tam, Nora Fung-Yee
    Xu, Steven Jing-Liang
    Lee, Chak-Lam
    Lee, Fred Wang-Fat
    MARINE POLLUTION BULLETIN, 2024, 209
  • [28] Molecular mechanism of glucose-6-phosphate utilization in the dinoflagellate Karenia mikimotoi
    Zhang, Chao
    Luo, Hao
    Huang, Liangmin
    Lin, Senjie
    HARMFUL ALGAE, 2017, 67 : 74 - 84
  • [29] Polyamines control the growth of the fish-killing dinoflagellate Karenia mikimotoi in culture
    Nishibori, Naoyoshi
    Imai, Ichiro
    HARMFUL ALGAE, 2013, 29 : 10 - 13
  • [30] The responses of harmful dinoflagellate Karenia mikimotoi to simulated ocean acidification at the transcriptional level
    Zhang, Jiazhu
    Yang, Qiongying
    Liu, Qianlou
    Liu, Shuqi
    Zhu, Yue
    Yao, Jiang
    Wang, Hong
    Guan, Wanchun
    HARMFUL ALGAE, 2022, 111