Injectable hyperbranched PEG crosslinked hyaluronan hydrogel microparticles containing mir-99a-3p modified subcutaneous ADSCs-derived exosomes was beneficial for long-term treatment of osteoarthritis

被引：18

作者：

Yin, Zhaowei ^{[1
]}

Qin, Chaoren ^{[3
]}

Pan, Shaowei ^{[1
]}

Shi, Chen ^{[1
]}

Wu, Guanfu ^{[2
]}

Feng, Yan ^{[3
]}

Zhang, Jing ^{[2
]}

Yu, Ziyi ^{[2
]}

Liang, Bin ^{[1
]}

Gui, Jianchao ^{[3
]}

机构：

[1] Nanjing Med Univ, Nanjing Hosp 1, Dept Orthopaed, Nanjing 210006, Peoples R China

[2] Nanjing Tech Univ, Coll Chem Engn, State Key Lab Mat Oriented Chem Engn, 30 Puzhu South Rd, Nanjing 211816, Peoples R China

[3] Nanjing Med Univ, Nanjing Hosp 1, Sports Med & Joint Surg, Nanjing 210006, Peoples R China

来源：

MATERIALS TODAY BIO | 2023年 / 23卷

基金：

中国国家自然科学基金;

关键词：

Osteoarthritis; Mesenchymal stem cells; Exosomes; MicroRNA; Microfluidics; Hydrogel; INFRAPATELLAR FAT PAD; CELLS; MICROSPHERES; DELIVERY;

D O I：

10.1016/j.mtbio.2023.100813

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Exosomes (Exos) secreted by adipose-derived stem cells (ADSCs) have shown potential in alleviating osteoar-thritis (OA). Previous studies indicated that infrapatellar fat pad (IPFP) derived stem cells (IPFSCs) may be more suitable for the treatment of OA than subcutaneous adipose tissue (ScAT) derived stem cells (ScASCs). However, it remains unclear which type of Exos offers superior therapeutic benefit for OA. This study first compared the differences between Exos derived from IPFP stem cells (ExosIPFP) and ScAT stem cells (ExosScAT) in OA treatment. Results suggested that ExosIPFP significantly inhibit the degradation of cartilage extracellular matrix (ECM) than ExosScAT, following this, the differences in microRNA (miRNA) expression between the two types of Exos using small RNA sequencing were performed. Subsequently, miR-99 b-3p was chosen and over-expressed in ExosScAT (ExosScAT-99b -3p), both in vivo and in vitro experiments demonstrated its efficacy in inhibiting the expression of ADAMTS4, promoting the repair of the ECM in OA. Finally, microfluidic technology was performed to fabricate a hyaluronan-based hydrogel microparticles (HMPs) for encapsulating Exos (HMPs@exos), the injectability, sus-tained release of Exos and long-term therapeutic effect on OA were validated. In summary, these results suggest miR-99 b-3p regulates the degradation of cartilage ECM by targeting ADAMTS4, the upregulation of miR-99 b-3p in ExosScAT would enable them to exhibit comparable or even superior effectiveness to ExosIPFP for OA treatment, making it a promising approach for OA treatment. Considering the abundant resources of ScAT and the limited availability of IPFP, ScAT harvested through liposuction could be genetically engineered to yield Exos for OA treatment. Furthermore, the encapsulation of Exos in HMPs provides an injectable sustained local drug release system, which could potentially enhance the efficacy of Exos and hold potential as future therapeutic strategies.

引用

页数：17