Molecular Taxonomy of South Africa's Catsharks: How Far Have We Come?

被引：3

作者：

van Staden, Michaela ^{[1
]}

Ebert, David A. ^{[2
,3
,4
]}

Gennari, Enrico ^{[3
,5
,6
]}

Leslie, Rob W. ^{[6
,7
]}

McCord, Meaghen E. ^{[8
,9
]}

Parkinson, Matthew ^{[3
,6
]}

Watson, Ralph G. A. ^{[6
,10
]}

Wintner, Sabine ^{[11
,12
]}

da Silva, Charlene ^{[13
]}

Bester-van der Merwe, Aletta E. ^{[1
]}

机构：

[1] Stellenbosch Univ, Dept Genet, ZA-7602 Cape Town, South Africa

[2] Pacific Shark Res Ctr, Moss Landing Marine Labs, Moss Landing, CA 95039 USA

[3] South African Inst Aquat Biodivers, ZA-6140 Makhanda, South Africa

[4] Calif Acad Sci, Dept Ichthyol, San Francisco, CA 94118 USA

[5] Oceans Res Inst, ZA-6500 Mossel Bay, South Africa

[6] Rhodes Univ, Dept Ichthyol & Fisheries Sci, ZA-6140 Makhanda, South Africa

[7] Univ Cape Town, Marine Res Inst, ZA-7700 Cape Town, South Africa

[8] South African Shark Conservancy, ZA-7200 Hermanus, South Africa

[9] Canadian Pk & Wilderness Soc, Vancouver, BC V6A 2T2, Canada

[10] Dyer Isl Conservat Trust, ZA-7220 Gansbaai, South Africa

[11] KwaZulu Natal Sharks Board, ZA-4320 Durban, South Africa

[12] Univ KwaZulu Natal, Sch Life Sci, ZA-4000 Durban, South Africa

[13] Dept Environm Forestry & Fisheries, ZA-8012 Cape Town, South Africa

来源：

DIVERSITY-BASEL | 2023年 / 15卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

Pentanchidae; Scyliorhinidae; conservation; DNA barcoding; Sanger sequencing; double peaks; heteroplasmy; introgression; MITOCHONDRIAL-DNA; SPECIES DELIMITATION; BLACKTIP SHARK; IDENTIFICATION; CHONDRICHTHYES; CARCHARHINIFORMES; REDESCRIPTION; DIVERSITY; BARCODE; RAYS;

D O I：

10.3390/d15070828

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The ability to correctly identify specimens at the species level is crucial for assessing and conserving biodiversity. Despite this, species-specific data are lacking for many of South Africa's catsharks due to a high level of morphological stasis. As comprehensive and curated DNA reference libraries are required for the reliable identification of specimens from morphologically similar species, this study reviewed and contributed to the availability of cytochrome c oxidase subunit I (COI) and nicotinamide adenine dehydrogenase subunit 2 (NADH2) sequences for South Africa's catsharks. A molecular taxonomic approach, implementing species delimitation and specimen assignment methods, was used to assess and highlight any taxonomic uncertainties and/or errors in public databases. The investigated species were summarised into 47 molecular operational taxonomic units (MOTUs), with some conflicting specimen assignments. Two Apristurus specimens sampled in this study remained unidentified, revealing the presence of previously undocumented genetic diversity. In contrast, haplotype sharing within Haploblepharus-attributed to nucleotide ambiguities-resulted in the delimitation of three congeners into a single MOTU. This study reveals that molecular taxonomy has the potential to flag undocumented species and/or misidentified specimens, and further highlights the need to implement integrated taxonomic assessments on catsharks that represent an irreplaceable component of biodiversity in the region.

引用

页数：16

共 106 条

[21] The hitchhiker's guide to single-locus species delimitation [J].