Enhancement of Lipid Signals in Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry with Ammonium Fluoride as a Matrix Additive

被引:2
作者
Holbrook, Joseph H. H. [1 ]
Sekera, Emily R. R. [2 ]
Lopez, Arbil [2 ]
Fries, Brian D. D. [2 ]
Tobias, Fernando [2 ]
Akkaya, Kubra [3 ]
Mihaylova, Maria M. M. [1 ,3 ,4 ]
Hummon, Amanda B. B. [1 ,2 ,4 ]
机构
[1] Ohio State Univ, Ohio State Biochem Program, Columbus, OH 43210 USA
[2] Ohio State Univ, Dept Chem & Biochem, Columbus, OH 43210 USA
[3] Ohio State Univ, Dept Biol Chem & Pharmacol, Columbus, OH 43210 USA
[4] Ohio State Univ, Comprehens Canc Ctr, Columbus, OH 43210 USA
基金
美国国家卫生研究院;
关键词
ELECTROSPRAY-IONIZATION;
D O I
10.1021/acs.analchem.3c00753
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Lipids are essential macromoleculesthat play a crucialrole innumerous biological events. Lipids are structurally diverse whichallows them to fulfill multiple functional roles. Matrix-assistedlaser desorption/ionization mass spectrometry imaging (MALDI-MSI)is a powerful tool to understand the spatial localization of lipidswithin biological systems. Herein, we report the use of ammonium fluoride(NH4F) as a comatrix additive to enhance lipid detectionin biological samples, with a signal increase of up to 200%. Emphasiswas placed on anionic lipid enhancement with negative polarity measurements,with some preliminary work on cationic lipids detailed. We observedlipid signal enhancement of [M-H](-) ions withthe addition of NH4F additive attributed to a proton transferreaction in several different lipid classes. Overall, our study demonstratesthat the use of the NH4F comatrix additive substantiallyimproves sensitivity for lipid detection in a MALDI system and iscapable of being applied to a variety of different applications.
引用
收藏
页码:10603 / 10609
页数:7
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