A Novel Linear B-Cell Epitope on the P54 Protein of African Swine Fever Virus Identified Using Monoclonal Antibodies

被引：6

作者：

Zheng, Nannan ^{[1
,2
,3
]}

Li, Chao ^{[1
,2
,3
]}

Hou, Haoyu ^{[1
,2
,3
]}

Chen, Yinlong ^{[1
,2
,3
]}

Zhang, Angke ^{[1
,2
,3
]}

Han, Shichong ^{[1
,2
,3
]}

Wan, Bo ^{[1
,2
,3
]}

Wu, Yanan ^{[1
,2
,3
]}

He, Hua ^{[1
,2
,3
]}

Wang, Na ^{[1
,2
,3
]}

Du, Yongkun ^{[1
,2
,3
]}

机构：

[1] Henan Agr Univ, Coll Vet Med, Zhengzhou 450046, Peoples R China

[2] Henan Agr Univ, Coll Vet Med, Int Joint Res Ctr Natl Anim Immunol, Zhengzhou 450046, Peoples R China

[3] Henan Engn Lab Anim Biol Prod, Zhengzhou 450046, Peoples R China

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 04期

基金：

中国博士后科学基金;

关键词：

African swine fever virus; P54; vaccine development; B-cell epitope; monoclonal antibody; PIGS; SERODIAGNOSIS; REPLICATION; P72;

D O I：

10.3390/v15040867

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The African swine fever virus (ASFV) is a highly infectious viral pathogen that presents a major threat to the global pig industry. No effective vaccine is available for the virus. The p54 protein, a major structural component of ASFV, is involved in virus adsorption and entry to target cells and also plays a key role in ASFV vaccine development and disease prevention. Here, we generated species-specific monoclonal antibodies (mAbs), namely 7G10A7F7, 6E8G8E1, 6C3A6D12, and 8D10C12C8 (subtype IgG1/kappa type), against the ASFV p54 protein and characterized the specificity of these mAbs. Peptide scanning techniques were used to determine the epitopes that are recognized by the mAbs, which defined a new B-cell epitope, TMSAIENLR. Amino acid sequence comparison showed that this epitope is conserved among all reference ASFV strains from different regions of China, including the widely prevalent, highly pathogenic strain Georgia 2007/1 (NC_044959.2). This study reveals important signposts for the design and development of ASFV vaccines and also provides critical information for the functional studies of the p54 protein via deletion analysis.

引用

页数：12

共 36 条

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