Polymorphisms in the MBL2 gene are associated with the plasma levels of MBL and the cytokines IL-6 and TNF-α in severe COVID-19

被引：13

作者：

Queiroz, Maria Alice Freitas ^{[1
,2
]}

Santiago, Angelica Menezes ^{[1
,3
]}

Brito, Wandrey Roberto dos Santos ^{[1
,2
]}

Pereira, Keise Adrielle Santos ^{[1
,2
]}

de Brito, William Botelho ^{[1
,3
]}

Torres, Maria Karoliny da Silva ^{[1
,2
]}

Lopes, Jeferson da Costa ^{[1
,2
]}

Santos, Erika Ferreira dos ^{[2
,4
]}

da Costa, Flavia Povoa ^{[2
,4
]}

de Sarges, Kevin Matheus Lima ^{[2
,4
]}

Cantanhede, Marcos Henrique Damasceno ^{[2
,4
]}

de Brito, Mioni Thieli Figueiredo Magalhaes ^{[4
]}

da Silva, Andrea Luciana Soares ^{[4
]}

Leite, Mauro de Meira ^{[4
]}

do Socorro de Almeida Viana, Maria de Nazare ^{[2
,4
]}

Rodrigues, Fabiola Brasil Barbosa ^{[2
,4
]}

da Silva, Rosilene ^{[2
,4
]}

Viana, Giselle Maria Rachid ^{[5
]}

Souza Chaves, Tania do Socorro ^{[5
,6
]}

Lameira Verissimo, Adriana de Oliveira ^{[7
]}

Carvalho, Mayara da Silva ^{[7
]}

Henriques, Daniele Freitas ^{[8
]}

dos Santos, Carla Pinheiro ^{[8
]}

Nunes, Juliana Abreu Lima ^{[9
]}

Costa, Iran Barros ^{[3
,9
]}

Graca Amoras, Ednelza da Silva ^{[1
]}

Lima, Sandra Souza ^{[1
]}

Cayres-Vallinoto, Izaura Maria Vieira ^{[1
,2
]}

Brasil-Costa, Igor ^{[3
,9
]}

Quaresma, Juarez Antonio Simoes ^{[10
]}

Falcao, Luiz Fabio Magno ^{[10
]}

dos Santos, Eduardo Jose Melo ^{[2
,4
]}

Vallinoto, Antonio Carlos Rosario ^{[1
,2
]}

机构：

[1] Fed Univ UFPA, Inst Biol Sci, Lab Virol, Belem, Brazil

[2] Fed Univ UFPA, Inst Biol Sci, Grad Program Biol Infect & Parasit Agents, Belem, Brazil

[3] Minist Hlth Brazil, Evandro Chagas Inst, Grad Program Virol, Dept Sci & Technol Innovat Strateg Hlth Inputs, Ananindeua, Brazil

[4] Fed Univ, Inst Biol Sci, Lab Genet Complex Dis, Belem, Brazil

[5] Evandro Chagas Inst, Parasitol Sect, Lab Basic Res Malaria, Hlth & Environm Surveillance Secretariat, Ananindeua, Brazil

[6] Fed Univ, Inst Med Sci, Sch Med, Belem, Para, Brazil

[7] Belem Adventist Hosp, Belem, Brazil

[8] Inst Evandro Chagas, Lab Immunol Sect Virol, Hlth & Environm Arbovirol & Hemorrhag Fevers Sect, Ananindeua, Brazil

[9] Brazilian Minist Hlth, Brazilian Minist Hlth, Lab Immunol, Sect Virol, Ananindeua, Brazil

[10] Univ State, Ctr Biol & Hlth Sci, Belem, Brazil

来源：

FRONTIERS IN IMMUNOLOGY | 2023年 / 14卷

关键词：

COVID-19; long COVID; MBL; polymorphisms; cytokines; MANNOSE-BINDING LECTIN; SARS-COV-2; IMPACT;

D O I：

10.3389/fimmu.2023.1151058

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IntroductionMannose-binding lectin (MBL) promotes opsonization, favoring phagocytosis and activation of the complement system in response to different microorganisms, and may influence the synthesis of inflammatory cytokines. This study investigated the association of MBL2 gene polymorphisms with the plasma levels of MBL and inflammatory cytokines in COVID-19. MethodsBlood samples from 385 individuals (208 with acute COVID-19 and 117 post-COVID-19) were subjected to real-time PCR genotyping. Plasma measurements of MBL and cytokines were performed by enzyme-linked immunosorbent assay and flow cytometry, respectively. ResultsThe frequencies of the polymorphic MBL2 genotype (OO) and allele (O) were higher in patients with severe COVID-19 (p< 0.05). The polymorphic genotypes (AO and OO) were associated with lower MBL levels (p< 0.05). IL-6 and TNF-alpha were higher in patients with low MBL and severe COVID-19 (p< 0.05). No association of polymorphisms, MBL levels, or cytokine levels with long COVID was observed. DiscussionThe results suggest that, besides MBL2 polymorphisms promoting a reduction in MBL levels and therefore in its function, they may also contribute to the development of a more intense inflammatory process responsible for the severity of COVID-19.

引用

页数：10

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