Extraction and characterization of hyaluronic acid from the eyeball of Nile Tilapia (Oreochromis niloticus)

被引:26
作者
Alcantara, Lyndervan Oliveira [1 ]
de Sousa, Juliana Rabelo [1 ]
Andrade, Fabia Karine [2 ]
Teixeira, Edson Holanda [3 ]
Cerqueira, Miguel Angelo [4 ]
da Silva, Andre Luis Coelho [5 ]
Souza Filho, Men de sa Moreira [6 ]
de Souza, Bartolomeu Warlene Silva [1 ]
机构
[1] Univ Fed Ceara, Dept Fishing Engn, Campus Pici 825, BR-60356000 Fortaleza, CE, Brazil
[2] Univ Fed Ceara, Dept Chem Engn, Grad Program Chem Engn, Campus Pici 709, BR-60455760 Fortaleza, CE, Brazil
[3] Fed Univ Ceara UFC, Fac Med, Dept Pathol & Forens Med, BR-60430160 Fortaleza, CE, Brazil
[4] Int Iberian Nanotechnol Lab, Ave Mestre Jose Veiga s-n, P-4715330 Braga, Portugal
[5] Univ Fed Ceara, Dept Biochem & Mol Biol, Campus Pici 907, BR-60451970 Fortaleza, CE, Brazil
[6] Embrapa Trop Agroind, Rua Dr Sara Mesquita, 2270, Planalto Pici, BR-60511110 Fortaleza, CE, Brazil
关键词
Oreochromis niloticus; Eyeball; Hyaluronic acid; Value aggregation; THERMAL-DEGRADATION; CHONDROITIN SULFATE; MOLECULAR-WEIGHT; BROAD RANGE; SURFACE; FISH; PURIFICATION; MICROWAVE; HYDROGELS; DELIVERY;
D O I
10.1016/j.ijbiomac.2022.12.016
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hyaluronic acid (HA) is a biopolymer of enormous value aggregation for in general industry. The vitreous humor of the eyeball from Nile tilapia contains appreciable amounts of hyaluronic acid. In this sense, the aim of this work was to extract and characterize hyaluronic acid from the eyeball of the Nile tilapia for biomedical appli-cations, adding value to fish industry residues. The characterization by infra-red (FTIR), 13C nuclear magnetic resonance (NMR) and high performance liquid chromatography (HPLC) confirmed that hyaluronic acid was obtained. The gel permeation chromatography (GPC) showed that the obtained material presents a low mo-lecular mass (37 KDa). Thermogravimetry (TGA), differential scanning calorimetry (DSC), X-ray diffraction (XRD) analysis showed that the materials present a thermal stability superior to the commercial hyaluronic acid from Streptococcus equi , with a partially crystalline character. The cytotoxicity assay (MTT method) with fibro-blast cells (L929) demonstrated that the extracted biopolymer besides not being cytotoxic, was able to stimulate cell proliferation. Therefore, the hyaluronic acid extracted from this source of residue constitutes a product with biotechnological potential, which has adequate quality for wide biomedical applications.
引用
收藏
页码:172 / 183
页数:12
相关论文
共 72 条
[1]   Hyaluronic acid and Chondroitin sulfate from marine and terrestrial sources: Extraction and purification methods [J].
Abdallah, Maha M. ;
Fernandez, Naiara ;
Matias, Ana A. ;
Bronze, Maria do Rosario .
CARBOHYDRATE POLYMERS, 2020, 243
[2]   Synthesis of novel grafted hyaluronic acid with antitumor activity [J].
Abu Elella, Mahmoud H. ;
Mohamed, Riham R. ;
Sabaa, Magdy W. .
CARBOHYDRATE POLYMERS, 2018, 189 :107-114
[3]   Improvement of the extraction procedure for hyaluronan from fish eyeball and the molecular characterization [J].
Amagai, Ichika ;
Tashiro, Yuri ;
Ogawa, Hiroo .
FISHERIES SCIENCE, 2009, 75 (03) :805-810
[4]   Layer-by-layer assembly of hyaluronic acid/carboxymethylchitosan polyelectrolytes on the surface of aminated mesoporous silica for the oral delivery of 5-fluorouracil [J].
Anirudhan, Thayyath S. ;
Vasantha, Chithra S. ;
Sasidharan, Athira V. .
EUROPEAN POLYMER JOURNAL, 2017, 93 :572-589
[5]  
Bainbridge P, 2013, J WOUND CARE, V22, P407
[6]   Self-healable hyaluronic acid/chitosan polyelectrolyte complex hydrogels and multilayers [J].
Barroso, Nagore ;
Guaresti, Olatz ;
Perez-Alvarez, Leyre ;
Ruiz-Rubio, Leire ;
Gabilondo, Nagore ;
Luis Vilas-Vilela, Jose .
EUROPEAN POLYMER JOURNAL, 2019, 120
[7]   Structural macromolecules and supramolecular organisation of the vitreous gel [J].
Bishop, PN .
PROGRESS IN RETINAL AND EYE RESEARCH, 2000, 19 (03) :323-344
[8]  
[陈胜军 Chen Shengjun], 2014, [核农学报, Journal of Nuclear Agricultural Sciences], V28, P1446
[9]   Degradation of hyaluronic acid powder by electron beam irradiation, gamma ray irradiation, microwave irradiation and thermal treatment: A comparative study [J].
Choi, Jong-il ;
Kim, Jae-Kyung ;
Kim, Jae-Hun ;
Kweon, Dong-Keon ;
Lee, Ju-Woon .
CARBOHYDRATE POLYMERS, 2010, 79 (04) :1080-1085
[10]   Microbial hyaluronic acid production [J].
Chong, BF ;
Blank, LM ;
Mclaughlin, R ;
Nielsen, LK .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2005, 66 (04) :341-351