Targeted metabolomic and transcriptomic reveal the regulation of UV-C on phenolics biosynthesis of peach fruit during storage

被引：7
作者：
Han, Shuai [1 ]
Cai, Hongfang [2 ]
Yu, Haitao [3 ]
Yu, Zhifang [3 ]
Wu, Youzhi [1 ]
机构：
[1] Shanghai Zhongqiao Vocat & Tech Univ, Coll Food & Drug, 3888 Caolang Rd, Shanghai 201514, Peoples R China
[2] Changshu Inst Technol, Coll Biol & Food Engn, 99 South Third Ring Rd, Changshu 215500, Peoples R China
[3] Nanjing Agr Univ, Coll Food Sci & Technol, 1 Weigang, Nanjing 210095, Jiangsu, Peoples R China
来源：
LWT-FOOD SCIENCE AND TECHNOLOGY | 2023年 / 190卷
关键词：
Prunus persica; Targeted metabolomic; Transcriptomic; UV; -C; Phenolics; PRUNUS-PERSICA; POLYPHENOLS; FLAVONOIDS; STRINGTIE; GENES; HISAT;
D O I：
10.1016/j.lwt.2023.115573
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Phenolic compounds are important secondary metabolites in peach fruit and play positive roles in plant resil-ience and human diets. To investigate the regulation of Ultraviolet-C (UV-C) irradiation on phenolics biosyn-thesis of peach fruit, the targeted metabolomic and transcriptomic were used to analyze polyphenolic metabolites and their gene expression patterns. The metabolomic results showed that a total of 45 phenolics were identified in the peach pulp. Total phenolic content was significantly increased by UV-C irradiation and 35 individual phenolics responded observably to UV-C treatment. In particular, UV-C irradiation dramatically increased the content of 2,6-dihydroxybenzoic acid, luteolin, quercitrin, astragalin, narcissin, nicotiflorin, sorhamnetin-3-O-glucoside and hesperidin. The HCT1, CYP98A1/3, COMT1 and CHS2/3 genes in phenyl-propanoid metabolic pathway and the CHI1/3, F3 ' H1/3, P2 ' GT1/2/3/4, UFGT3 and DFR2/3 genes in flavonoid metabolic pathway were significantly regulated by UV-C irradiation. Overall, the present study revealed that phenolics biosynthesis is regulated by UV-C irradiation at the level of metabolites and molecule supported by targeted metabolomic and transcriptomic results.
引用
收藏
页数：14
相关论文
共 27 条
[1]   Computational Studies of Free Radical-Scavenging Properties of Phenolic Compounds [J].
Alov, Petko ;
Tsakovska, Ivanka ;
Pajeva, Ilza .
CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2015, 15 (02) :85-104
[2]   Polyphenols: A Diverse Class of Multi-Target Anti-HIV-1 Agents [J].
Andrae-Marobela, Kerstin ;
Ghislain, Fotso Wabo ;
Okatch, Harriet ;
Majinda, Runner R. T. .
CURRENT DRUG METABOLISM, 2013, 14 (04) :392-413
[3]   Phenolic and carotenoid composition of canned peaches (Prunus persica) and apricots (Prunus armeniaca) as affected by variety and peeling [J].
Campbell, Oluranti E. ;
Padilla-Zakour, Olga I. .
FOOD RESEARCH INTERNATIONAL, 2013, 54 (01) :448-455
[4]   fastp: an ultra-fast all-in-one FASTQ preprocessor [J].
Chen, Shifu ;
Zhou, Yanqing ;
Chen, Yaru ;
Gu, Jia .
BIOINFORMATICS, 2018, 34 (17) :884-890
[5]   LC-ESI-QTOF/MS Characterisation of Phenolic Acids and Flavonoids in Polyphenol-Rich Fruits and Vegetables and Their Potential Antioxidant Activities [J].
Gu, Chunhe ;
Howell, Kate ;
Dunshea, Frank R. ;
Suleria, Hafiz A. R. .
ANTIOXIDANTS, 2019, 8 (09)
[6]   Polyphenol metabolic diversity of Chinese peach and nectarine at thinned and ripe stages by UPLC-ESI-Q-TOF-MS combined with multivariate statistical analysis [J].
Guo, Chongting ;
Bi, Jinfeng ;
Li, Xuan ;
Lyu, Jian ;
Wu, Xinye ;
Xu, Ye .
JOURNAL OF FOOD COMPOSITION AND ANALYSIS, 2020, 90
[7]   Effect of nitric oxide on sugar metabolism in peach fruit (cv. Xiahui 6) during cold storage [J].
Han, Shuai ;
Cai, Hongfang ;
An, Xiujuan ;
Huan, Chen ;
Wu, Xiaoqin ;
Jiang, Li ;
Yu, Mingliang ;
Ma, Ruijuan ;
Yu, Zhifang .
POSTHARVEST BIOLOGY AND TECHNOLOGY, 2018, 142 :72-80
[8]   The Adaptive Role of Flavonoids: Emphasis on Cereals [J].
Khlestkina, E. K. .
CEREAL RESEARCH COMMUNICATIONS, 2013, 41 (02) :185-198
[9]  
Kim D, 2015, NAT METHODS, V12, P357, DOI [10.1038/NMETH.3317, 10.1038/nmeth.3317]
[10]   RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome [J].
Li, Bo ;
Dewey, Colin N. .
BMC BIOINFORMATICS, 2011, 12
123