CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

被引：25

作者：

Su, Weiran ^{[1
,2
,3
]}

Li, Junru ^{[1
,2
,3
]}

Ji, Chen ^{[1
,2
,3
]}

Chen, Congshuo ^{[1
,2
,3
]}

Wang, Yuzheng ^{[1
,2
,3
]}

Dai, Huili ^{[1
,2
,3
]}

Li, Fengqin ^{[1
,2
,3
]}

Liu, Peifeng ^{[1
,2
,3
]}

机构：

[1] Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Canc Inst, Sch Med,State Key Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China

[2] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Cent Lab, Shanghai 200127, Peoples R China

[3] Shanghai Jiao Tong Univ, Renji Hosp, Micronano Res & Diag Ctr, Sch Med, Shanghai 200127, Peoples R China

来源：

NANO RESEARCH | 2023年 / 16卷 / 07期

基金：

中国国家自然科学基金;

关键词：

clustered regularly interspaced short palindromic repeats (CRISPR); CRISPR-associated (Cas); detection; nucleic acid targets; non-nucleic acid targets; DNA CLEAVAGE; EVOLUTIONARY CLASSIFICATION; CRYSTAL-STRUCTURE; CAS SYSTEMS; GUIDE RNA; AMPLIFICATION; COMPLEX; CPF1; TRANSCRIPTION; ENDONUCLEASE;

D O I：

10.1007/s12274-023-5567-4

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis-cleavage and nonspecific trans-cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.

引用

页码：9940 / 9953

页数：14

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