CRISPR/Cas systems for the detection of nucleic acid and non-nucleic acid targets

被引：25
|
作者：
Su, Weiran [1 ,2 ,3 ]
Li, Junru [1 ,2 ,3 ]
Ji, Chen [1 ,2 ,3 ]
Chen, Congshuo [1 ,2 ,3 ]
Wang, Yuzheng [1 ,2 ,3 ]
Dai, Huili [1 ,2 ,3 ]
Li, Fengqin [1 ,2 ,3 ]
Liu, Peifeng [1 ,2 ,3 ]
机构：
[1] Shanghai Jiao Tong Univ, Renji Hosp, Shanghai Canc Inst, Sch Med,State Key Lab Oncogenes & Related Genes, Shanghai 200032, Peoples R China
[2] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Cent Lab, Shanghai 200127, Peoples R China
[3] Shanghai Jiao Tong Univ, Renji Hosp, Micronano Res & Diag Ctr, Sch Med, Shanghai 200127, Peoples R China
来源：
NANO RESEARCH | 2023年 / 16卷 / 07期
基金：
中国国家自然科学基金;
关键词：
clustered regularly interspaced short palindromic repeats (CRISPR); CRISPR-associated (Cas); detection; nucleic acid targets; non-nucleic acid targets; DNA CLEAVAGE; EVOLUTIONARY CLASSIFICATION; CRYSTAL-STRUCTURE; CAS SYSTEMS; GUIDE RNA; AMPLIFICATION; COMPLEX; CPF1; TRANSCRIPTION; ENDONUCLEASE;
D O I：
10.1007/s12274-023-5567-4
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems are becoming powerful tools for disease biomarkers detection. Due to the specific recognition, cis-cleavage and nonspecific trans-cleavage capabilities, CRISPR/Cas systems have implemented the detection of nucleic acid targets (DNA and RNA) as well as non-nucleic acid targets (e.g., proteins, exosomes, cells, and small molecules). In this review, we first summarize the principles and characteristics of various CRISPR/Cas systems, including CRISPR/Cas9, Cas12, Cas13 and Cas14 systems. Then, various types of applications of CRISPR/Cas systems used in detecting nucleic and non-nucleic acid targets are introduced emphatically. Finally, the prospects and challenges of their applications in biosensing are discussed.
引用
收藏
页码：9940 / 9953
页数：14
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