Development of an ARMS multiplex real-time PCR assay for the detection of HLA-B*13:01 genotype by detecting highly specific SNPs

被引：1

作者：

Yao, Hao ^{[1
]}

Wang, Kaixuan ^{[1
]}

Lu, Sihai ^{[1
]}

Cao, Fang ^{[2
]}

Dai, Penggao ^{[1
,3
]}

机构：

[1] Northwest Univ Xian, Coll Life Sci, Natl Engn Res Ctr Miniaturized Detect Syst, Xian, Peoples R China

[2] Shaanxi Prov Canc Hosp, Dept Clin Pharm, Xian, Shaanxi, Peoples R China

[3] Northwest Univ Xian, Sch Life Sci, Xian 710069, Peoples R China

来源：

PHARMACOGENETICS AND GENOMICS | 2024年 / 34卷 / 02期

关键词：

ARMS multiplex real-time PCR; dapsone hypersensitivity syndrome; ethnic groups; HLA-B*13:01; HLA-B; HYPERSENSITIVITY REACTIONS; LEPROSY PATIENTS; DAPSONE;

D O I：

10.1097/FPC.0000000000000517

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Objectives HLA-B*13:01 was strongly associated with Dapsone Hypersensitivity Syndrome (DHS). This study aimed to develop and validate a rapid and economical method for HLA-B*13:01 genotyping.Methods Two tubes multiplex real-time PCR detection system comprising amplification refractory mutation system primers and TaqMan probes was established for HLA-B*13:01 genotyping. Sequence-based typing was applied to validate the accuracy of the assay.Results The accuracy of the assay was 100% for HLA-B*13:01 genotyping. The detection limit of the new method was 0.025 ng DNA. The positive rate of HLA-B*13:01 in the Bouyei (20%, n = 50) populations was significantly higher than that in the Uighur population (4%, n = 100), Han (4.5%, n = 200), and Tibetan (1%, n = 100) (P < 0.05).Conclusion The proposed method is rapid and reliable for HLA-B*13:01 screening in a clinical setting.

引用

页码：53 / 59

页数：7