Autologous dendritic cell vaccination against HIV-1 induces changes in natural killer cell phenotype and functionality

被引：9

作者：

Laeremans, Thessa ^{[1
]}

den Roover, Sabine ^{[1
]}

Lungu, Cynthia ^{[2
]}

D'haese, Sigrid ^{[1
]}

Gruters, Rob A. A. ^{[2
]}

Allard, Sabine D. D. ^{[3
,4
]}

Aerts, Joeri L. L. ^{[1
]}

机构：

[1] Vrije Univ Brussel, Neuroaging & Viro Immunotherapy Res Grp, Brussels, Belgium

[2] Erasmus MC, Dept Virosci, Rotterdam, Netherlands

[3] Univ Ziekenhuis Brussel, Dept Internal Med & Infect Dis, Brussels, Belgium

[4] Vrije Univ Brussel, Brussels, Belgium

来源：

NPJ VACCINES | 2023年 / 8卷 / 01期

关键词：

NK CELLS; T-CELLS; RESPONSES; MEMORY; RECEPTORS; SUBSET; ACTIVATION; EXPRESSION; ANTIBODY; DISEASE;

D O I：

10.1038/s41541-023-00631-z

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Although natural killer (NK) cells have been studied in connection with dendritic cell (DC)-based vaccination in the field of cancer immunology, their role has barely been addressed in the context of therapeutic vaccination against HIV-1. In this study, we evaluated whether a therapeutic DC-based vaccine consisting of monocyte-derived DCs electroporated with Tat, Rev and Nef encoding mRNA affects NK cell frequency, phenotype and functionality in HIV-1-infected individuals. Although the frequency of total NK cells did not change, we observed a significant increase in cytotoxic NK cells following immunisation. In addition, significant changes in the NK cell phenotype associated with migration and exhaustion were observed together with increased NK cell-mediated killing and (poly)functionality. Our results show that DC-based vaccination has profound effects on NK cells, which highlights the importance of evaluating NK cells in future clinical trials looking at DC-based immunotherapy in the context of HIV-1 infection.

引用

页数：13

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