Anti-angiogenic effect of nano-formulated water soluble kaempferol and combretastatin in an in vivo chick chorioallantoic membrane model and HUVEC cells

被引：6
作者：
Subbaraj, Gowtham Kumar [1 ,2 ,9 ,10 ]
Masoodi, Tariq [3 ]
Yasam, Santhosh Kumar [1 ,2 ]
Chandrashekar, Kirubhanand [4 ]
Kulanthaivel, Langeswaran [5 ]
Shaik, Noor Ahmad [6 ]
Hashem, Sheema [7 ]
Akil, Ammira S. Alshabeeb [8 ]
Bhat, Ajaz A. [8 ]
机构：
[1] Chettinad Hosp, Fac Allied Hlth Sci, Kelambakkam 603103, India
[2] Chettinad Acad Res & Educ, Res Inst, Kelambakkam 603103, India
[3] Sidra Med, Lab Canc Immunol & Genet, Doha, Qatar
[4] All India Inst Med Sci AIIMS, Dept Anat, Nagpur, Maharashtra, India
[5] Alagappa Univ, Dept Biotechnol, Canc Genet & Mol Biol Lab, Sci Campus, Karaikkudi 630003, Tamil Nadu, India
[6] King Abdulaziz Univ, Fac Med, Dept Genet Med, Jeddah 21589, Saudi Arabia
[7] Sidra Med, Dept Human Genet, Doha, Qatar
[8] Sidra Med, Dept Human Genet Precis Med Diabet, Obes & Canc Res Program, Doha, Qatar
[9] Chettinad Hosp, Fac Allied Hlth Sci, Old Mahabalipuram Rd OMR, Kelambakkam 603103, Tamil Nadu, India
[10] Chettinad Acad Res & Educ, Res Inst, Old Mahabalipuram Rd OMR, Kelambakkam 603103, Tamil Nadu, India
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 163卷
关键词：
Angiogenesis; Chorioallantoic membrane; Nano-formulation; Kaempferol; Combretastatin; INHIBITS ANGIOGENESIS; EXPRESSION; CONDUCTANCE; FLAVONOIDS; GROWTH;
D O I：
10.1016/j.biopha.2023.114820
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The present study evaluated the efficacy of nano-formulated water-soluble kaempferol and combretastatin alone and combined against the native kaempferol and combretastatin on angiogenesis. The solvent evaporation method was used to synthesize the nano-formulated water-soluble kaempferol and combretastatin and characterized using various analyses such as dynamic light scattering (DLS) and Fourier-transform infrared (FT-IR) spectroscopy.The anti-angiogenic activity of native, nano-formulated water-soluble kaempferol and combretastatin was investigated by cell viability on HUVEC and A498 cell lines, while chick chorioallantoic membrane (CAM) assay was utilized to assess morphometric and histopathological changes, and mRNA expressions of VEGF-A and FGF2 using qRT-PCR. MTT assay results revealed that the combination of nano-formulated watersoluble kaempferol and combretastatin significantly reduced the cell viability compared to control, individual treatments of native, nano-formulated water-soluble kaempferol, and combretastatin. Morphometric analysis of CAM showed that treatment with nano-formulated water-soluble kaempferol and combretastatin caused a substantial decrease in density, vessel network, branch points, and nets of CAM blood vessels. The histopathological results of CAM showed the irregular shape of blood vessels at the thin stratum of chronic endoderm, and blood capillaries were diminished compared to the control. In addition, the mRNA expression levels of VEGF-A and FGF2 were significantly decreased compared with native forms. Therefore, the findings of this study indicate that nano-formulated water-soluble combretastatin and kaempferol suppress angiogenesis by preventing the activation of endothelial cells and suppressing factors of angiogenesis. Moreover, a combination of nano-formulated water-soluble kaempferol and combretastatin worked much better than individual treatments.
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页数：11
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