Impact of Multiple Vitrification-Warming Procedures and Insemination Methods on Pregnancy and Neonatal Outcomes in Preimplantation Genetic Testing for Aneuploidy

被引：2
作者：
Zhang, Qian [1 ,2 ,3 ,4 ,5 ,6 ]
Yu, Wenhao [1 ,2 ,3 ,4 ,5 ,6 ]
Jin, Chenxi [1 ,2 ,3 ,4 ,5 ,6 ]
Ni, Tianxiang [1 ,2 ,3 ,4 ,5 ,6 ]
Zhou, Tingting [1 ,2 ,3 ,4 ,5 ,6 ]
Zhao, Qing [1 ,2 ,3 ,4 ,5 ,6 ]
Wang, Weilin [1 ,2 ,3 ,4 ,5 ,6 ]
Li, Yunjia [7 ]
Yan, Junhao [1 ,2 ,3 ,4 ,5 ,6 ]
机构：
[1] Shandong Univ, Ctr Reprod Med, Jinan 250012, Shandong, Peoples R China
[2] Shandong Univ, Key Lab Reprod Endocrinol, Minist Educ, Jinan 250012, Shandong, Peoples R China
[3] Shandong Key Lab Reprod Med, Jinan 250012, Shandong, Peoples R China
[4] Shandong Prov Clin Res Ctr Reprod Hlth, Jinan 250012, Shandong, Peoples R China
[5] Shandong Technol Innovat Ctr Reprod Hlth, Jinan 250012, Shandong, Peoples R China
[6] Shandong Univ, Natl Res Ctr Assisted Reprod Technol & Reprod Gene, Jinan 250012, Shandong, Peoples R China
[7] Linyi Peoples Hosp, Linyi 276000, Shandong, Peoples R China
来源：
REPRODUCTIVE SCIENCES | 2023年 / 30卷 / 07期
基金：
中国国家自然科学基金;
关键词：
PGT-A; Vitrification; IVF; ICSI; Pregnancy and neonatal outcomes; INTRACYTOPLASMIC SPERM INJECTION; IN-VITRO FERTILIZATION; BLASTOCYST BIOPSY; EMBRYO-TRANSFER; COUPLES; INFERTILITY; CYCLES; IVF;
D O I：
10.1007/s43032-023-01177-0
中图分类号：
R71 [妇产科学];
学科分类号：
100211 ;
摘要：
This study was to determine whether multiple vitrification-warming procedures and insemination method are associated with pregnancy and neonatal outcomes in preimplantation genetic testing for aneuploidy (PGT-A). This was a retrospective, single-center, observational study of 112 patients who underwent standard PGT-A practice and 154 patients who desired PGT-A for their vitrified unbiopsied blastocysts. A total of 97 euploid blastocysts biopsied and vitrified-warmed once and 117 euploid blastocysts biopsied once but vitrified-warmed twice (83 in vitro fertilization [IVF]-derived and 34 intracytoplasmic sperm injection [ICSI]-derived euploid blastocysts) were transferred. The primary outcome was the blastocyst survival rate for transfer, live birth rate, and neonatal outcomes. The results showed that an additional vitrification-warming procedure on blastocysts resulted in a lower but not statistically different survival rate for transfer. Compared with euploid blastocysts vitrified-warmed once, those vitrified-warmed twice provided statistically similar live birth rate. Neonatal outcomes, including the sex ratio, gestational age, birthweight, preterm birth rate, and low birthweight rate, did not differ between single and double vitrification. No significant differences were observed in rates of blastocyst survival, blastocyst euploid and live birth, and neonatal outcomes resulting from either conventional IVF or ICSI. The neonatal follow-up of babies live-born so far did not report any congenital malformations. In conclusion, an additional vitrification-warming on blastocysts had no detectable adverse impact on clinical outcomes after frozen-thawed single euploid blastocyst transfer in PGT-A cases; and ICSI did not confer any benefit in improving clinical outcomes compared with conventional IVF in cases requiring PGT-A on already vitrified nonbiopsied blastocysts.
引用
收藏
页码：2302 / 2312
页数：11
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