Response surface methodology optimization and HPLC-ESI-QTOF-MS/MS analysis on ultrasonic-assisted extraction of phenolic compounds from okra (Abelmoschus esculentus) and their antioxidant activity

Okra (Abelmoschus esculentus) has attracted a growing attention for its nutritional and medicinal values, while few studies focused on systemic study of okra polyphenols (OP). In order to obtain the maximum extracted ef-ficiency, response surface methodology was used to optimize ultrasonic-assisted extraction conditions. The maximum TPC was 7.02 mg GAE/g dw under the condition of solid-liquid ratio 1:25, ethanol concentration 70 %, 40 min, and 142 W at 46 degrees C. Then 27 compounds in OP were identified by HPLC-ESI-QTOF-MS/MS, among which 7-hydroxycoumarin, scopoletin, luteolin and et al were firstly identified from okra. Furthermore, OP exhibited antioxidant activity in reducing power (FRAP, 9.77 mM Fe2+/g OP) and radical scavenging (DPPH, IC50 19.31 mu g/mL; SARC, IC50 210.81 mu g/ml). Moreover, OP significantly inhibited cell apoptosis and ROS generation, and alleviated oxidative damage in t-BHP induced HUVECs. Overall, our findings could provide perspective for further potential employments of okra as functional food.