Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies

被引:28
作者
Capetti, Caio Cesar de Mello [1 ]
Pellegrini, Vanessa Oliveira Arnoldi [1 ]
Santo, Melissa Cristina Espirito [1 ]
Cortez, Anelyse Abreu [1 ]
Falvo, Mauricio [1 ]
Curvelo, Antonio Aprigio da Silva [2 ]
Campos, Eleonora [3 ,4 ]
Filgueiras, Jefferson Gonsalves [5 ,6 ]
Guimaraes, Francisco Eduardo Gontijo [1 ]
de Azevedo, Eduardo Ribeiro [1 ]
Polikarpov, Igor [1 ]
机构
[1] Univ Sao Paulo, Inst Fis Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
[2] Univ Sao Paulo, Inst Quim Sao Carlos, Ave Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP, Brazil
[3] Inst Nacl Tecnol Agr INTA, CICVyA, Inst Biotecnol, Los Reseros & N Repetto, RA-1686 Hurlingham, Buenos Aires, Argentina
[4] Consejo Nacl Invest Cient & Tecn CONICET, Ciudad Autonoma, Buenos Aires, Argentina
[5] Univ Fed Fluminense, Inst Quim, Outeiro Sao Joao Batista, BR-24020007 Niteroi, RJ, Brazil
[6] Univ Fed Rio De Janeiro, Inst Fis, CP68528, BR-21941972 Rio De Janeiro, RJ, Brazil
基金
巴西圣保罗研究基金会;
关键词
Corn cobs; Xylooligosaccharides; Xylanase; Xylosidase; Hydrothermal pretreatment; Alkali pretreatment; XYLAN; HYDROLYSIS; BIOMASS; GH10;
D O I
10.1016/j.carbpol.2022.120174
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Corn cobs (CCs) are abundant xylan-rich agricultural wastes. Here, we compared CCs XOS yields obtained via two different pretreatment routs, alkali and hydrothermal, using a set of recombinant endo- and exo-acting enzymes from GH10 and GH11 families, which have different restrictions for xylan substitutions. Furthermore, impacts of the pretreatments on chemical composition and physical structure of the CCs samples were evaluated. We demonstrated that alkali pretreatment route rendered 59 mg of XOS per gram of initial biomass, while an overall XOS yield of 115 mg/g was achieved via hydrothermal pretreatment using a combination of GH10 and GH11 enzymes. These results hold a promise of ecologically sustainable enzymatic valorization of CCs via "green" and sustainable XOS production.
引用
收藏
页数:12
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