Nitrogen transfer from root exudates to the rhizobiome: A 15N stem feeding method

被引：1
|
作者：
De Sena, Aidan [1 ]
Madramootoo, Chandra A. [1 ]
Whalen, Joann K. [2 ]
机构：
[1] McGill Univ, Dept Bioresource Engn, 21,111 Lakeshore Rd,Macdonald Campus, Sainte Anne De Bellevue, PQ H9X 3V9, Canada
[2] McGill Univ, Dept Nat Resource Sci, 21,111 Lakeshore Rd,Macdonald Campus, St Anne De Dellevue, PQ H9X 3V9, Canada
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2023年 / 186卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
15N isotope tracing; Cotton-wick method; Leaf tip feeding method; Rhizodeposition; Soil microbiome; Rhizosphere; RHIZODEPOSITION; TOXICITY; SOIL;
D O I：
10.1016/j.soilbio.2023.109159
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Plant roots exude nitrogen (N)-containing compounds, which are assimilated by the rhizobiome to maintain stoichiometric homeostasis. Various 15N-tracing methods exist to estimate the rhizobiome-N derived from root exudation but have never been validated with a full factorial experiment. We exposed ryegrass (Lolium multi-florum) to 15N solutions through different plant organs (stem or leaf feeding), with different 15N-tracers (urea or NH4NO3) and concentrations (64.5, 129 or 193 mmol 15N L-1). Stem feeding with either 15N-source at the highest concentration was the only effective way to quantify root exudate-N assimilation by the rhizobiome, and we recommend this 15N-labeling method for the reliable assessment of microbiome-N dynamics in the rhizosphere.
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页数：4
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