Nitrogen transfer from root exudates to the rhizobiome: A 15N stem feeding method

被引：1
|
作者：
De Sena, Aidan [1 ]
Madramootoo, Chandra A. [1 ]
Whalen, Joann K. [2 ]
机构：
[1] McGill Univ, Dept Bioresource Engn, 21,111 Lakeshore Rd,Macdonald Campus, Sainte Anne De Bellevue, PQ H9X 3V9, Canada
[2] McGill Univ, Dept Nat Resource Sci, 21,111 Lakeshore Rd,Macdonald Campus, St Anne De Dellevue, PQ H9X 3V9, Canada
来源：
SOIL BIOLOGY & BIOCHEMISTRY | 2023年 / 186卷
基金：
加拿大自然科学与工程研究理事会;
关键词：
15N isotope tracing; Cotton-wick method; Leaf tip feeding method; Rhizodeposition; Soil microbiome; Rhizosphere; RHIZODEPOSITION; TOXICITY; SOIL;
D O I：
10.1016/j.soilbio.2023.109159
中图分类号：
S15 [土壤学];
学科分类号：
0903 ; 090301 ;
摘要：
Plant roots exude nitrogen (N)-containing compounds, which are assimilated by the rhizobiome to maintain stoichiometric homeostasis. Various 15N-tracing methods exist to estimate the rhizobiome-N derived from root exudation but have never been validated with a full factorial experiment. We exposed ryegrass (Lolium multi-florum) to 15N solutions through different plant organs (stem or leaf feeding), with different 15N-tracers (urea or NH4NO3) and concentrations (64.5, 129 or 193 mmol 15N L-1). Stem feeding with either 15N-source at the highest concentration was the only effective way to quantify root exudate-N assimilation by the rhizobiome, and we recommend this 15N-labeling method for the reliable assessment of microbiome-N dynamics in the rhizosphere.
引用
收藏
页数：4
相关论文
共 50 条
  • [1] Nitrogen transfer from a legume tree to the associated grass estimated by the isotopic signature of tree root exudates:: A comparison of the 15N leaf feeding and natural 15N abundance methods
    Sierra, J.
    Daudin, D.
    Domenach, A. -M.
    Nygren, P.
    Desfontaines, L.
    EUROPEAN JOURNAL OF AGRONOMY, 2007, 27 (2-4) : 178 - 186
  • [2] Effect of protein restriction on 15N transfer from dietary [15N]alanine and [15N]Spirulina platensis into urea
    Hamadeh, MJ
    Hoffer, LJ
    AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 2001, 281 (02): : E349 - E356
  • [3] Below-ground nitrogen transfer between different grassland species: Direct quantification by 15N leaf feeding compared with indirect dilution of soil 15N
    Henning Høgh-Jensen
    Jan K. Schjoerring
    Plant and Soil, 2000, 227 : 171 - 183
  • [4] Below-ground nitrogen transfer between different grassland species:: Direct quantification by 15N leaf feeding compared with indirect dilution of soil 15N
    Hogh-Jensen, H
    Schjoerring, JK
    PLANT AND SOIL, 2000, 227 (1-2) : 171 - 183
  • [5] Nitrogen isotopes in mammalian herbivores:: Hair δ15N values from a controlled feeding study
    Sponheimer, M
    Robinson, T
    Ayliffe, L
    Roeder, B
    Hammer, J
    Passey, B
    West, A
    Cerling, T
    Dearing, D
    Ehleringer, J
    INTERNATIONAL JOURNAL OF OSTEOARCHAEOLOGY, 2003, 13 (1-2) : 80 - 87
  • [6] Quantifying the fate of nitrogen from cereal rye root and shoot biomass using 15N
    Roth, Richard T.
    Lacey, Corey G.
    Camberato, James J.
    Armstrong, Shalamar D.
    NUTRIENT CYCLING IN AGROECOSYSTEMS, 2023, 125 (02) : 219 - 234
  • [7] Quantifying the fate of nitrogen from cereal rye root and shoot biomass using 15N
    Richard T. Roth
    Corey G. Lacey
    James J. Camberato
    Shalamar D. Armstrong
    Nutrient Cycling in Agroecosystems, 2023, 125 : 219 - 234
  • [8] 15N labeling technology reveals enhancement of nitrogen uptake and transfer by root interaction in cotton/soybean intercropping
    Jing, Bo
    Shi, Wenjuan
    Wang, Han
    Lin, Fengmei
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 2023, 103 (13) : 6307 - 6316
  • [9] δ15N as an integrator of the nitrogen cycle
    Robinson, D
    TRENDS IN ECOLOGY & EVOLUTION, 2001, 16 (03) : 153 - 162
  • [10] Quantifying the transfer of prey δ15N signatures into coral holobiont nitrogen pools
    Rangel, Maria Salome
    Erler, Dirk
    Tagliafico, Alejandro
    Cowden, Ken
    Scheffers, Sander
    Christidis, Les
    MARINE ECOLOGY PROGRESS SERIES, 2019, 610 : 33 - 49
12345