Ageing-associated small RNA cargo of extracellular vesicles

被引：7

作者：

Kern, Fabian ^{[1
,2
]}

Kuhn, Thomas ^{[3
,4
]}

Ludwig, Nicole ^{[5
,6
]}

Simon, Martin ^{[7
]}

Groeger, Laura ^{[5
]}

Fabis, Natalie ^{[7
]}

Aparicio-Puerta, Ernesto ^{[1
]}

Salhab, Abdulrahman ^{[8
]}

Fehlmann, Tobias ^{[1
]}

Hahn, Oliver ^{[9
]}

Engel, Annika ^{[1
]}

Wagner, Viktoria ^{[1
]}

Koch, Marcus ^{[10
]}

Winek, Katarzyna

Soreq, Hermona

Nazarenko, Irina ^{[11
]}

Fuhrmann, Gregor ^{[3
,4
]}

Wyss-Coray, Tony ^{[9
]}

Meese, Eckart ^{[5
]}

Keller, Verena ^{[1
]}

Laschke, Matthias W.

Keller, Andreas ^{[1
,2
]}

机构：

[1] Saarland Univ, Chair Clin Bioinformat, Saarland Informat Campus, D-66123 Saarbrucken, Germany

[2] Helmholtz Ctr Infect Res HZI, Helmholtz Inst Pharmaceut Res Saarland HIPS, Dept Clin Bioinformat, Saarbrucken, Germany

[3] Helmholtz Ctr Infect Res HZI, Helmholtz Inst Pharmaceut Res Saarland HIPS, Biogen Nanotherapeut Grp ION, Saarbrucken, Germany

[4] Saarland Univ, Dept Pharm, Saarbrucken, Germany

[5] Saarland Univ, Dept Human Genet, Homburg, Germany

[6] Saarland Univ, Ctr Human & Mol Biol, Homburg, Germany

[7] Wuppertal Univ, Mol Cell Biol & Microbiol, Wuppertal, Germany

[8] Saarland Univ, Dept Genet & Epigenet, Saarbrucken, Germany

[9] Stanford Univ, Dept Neurol & Neurol Sci, Stanford, CA USA

[10] INM Leibniz Inst New Mat, Saarbrucken, Germany

[11] Univ Freiburg, Med Ctr, Freiburg, Germany

来源：

RNA BIOLOGY | 2023年 / 20卷 / 01期

关键词：

Noncoding RNA; tissue ageing; extracellular vesicles; plasma; miRNA; STEM-CELLS; MICRORNAS; EXOSOME; TRANSCRIPTOME; METAANALYSIS; DICTIONARY; MIRPATHDB;

D O I：

10.1080/15476286.2023.2234713

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work on murine models and humans demonstrated global as well as tissue-specific molecular ageing trajectories of RNAs. Extracellular vesicles (EVs) are membrane vesicles mediating the horizontal transfer of genetic information between different tissues. We sequenced small regulatory RNAs (sncRNAs) in two mouse plasma fractions at five time points across the lifespan from 2-18 months: (1) sncRNAs that are free-circulating (fc-RNA) and (2) sncRNAs bound outside or inside EVs (EV-RNA). Different sncRNA classes exhibit unique ageing patterns that vary between the fcRNA and EV-RNA fractions. While tRNAs showed the highest correlation with ageing in both fractions, rRNAs exhibited inverse correlation trajectories between the EV- and fc-fractions. For miRNAs, the EV-RNA fraction was exceptionally strongly associated with ageing, especially the miR-29 family in adipose tissues. Sequencing of sncRNAs and coding genes in fat tissue of an independent cohort of aged mice up to 27 months highlighted the pivotal role of miR-29a-3p and miR-29b-3p in ageing-related gene regulation that we validated in a third cohort by RT-qPCR.

引用

页码：482 / 494

页数：13

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