A panel of visual bacterial biosensors for the rapid detection of genotoxic and oxidative damage: A proof of concept study

The emergence of new compounds during the past decade requires a high-throughput screening method for toxicity assay. The stress-responsive whole-cell biosensor is a powerful tool to evaluate direct or indirect damages of biological macromolecules induced by toxic chemicals. In this proof-of-concept study, nine well-characterized stress-responsive promoters were first selected to assemble a set of blue indigoidine-based biosensors. The PuspA- based, PfabA-based, and PgrpE-based biosensors were eliminated due to their high background. A dose -dependent increase of visible blue signal was observed in PrecA-, PkatG-, and PuvrA-based biosensors, respon-sive to potent mutagens, including mitomycin and nalidixic acid, but not to genotoxic lead and cadmium. The PrecA, PkatG, and Ppgi gene promoters were further fused to a purple deoxyviolacein synthetic enzyme cluster. Although high basal production of deoxyviolacein is unavoidable, an enhanced visible purple signal in response to mitomycin and nalidixic acid was observed as dose-dependent, especially in PkatG-based biosensors. The study shows that a set of stress-responsive biosensors employing visible pigment as a reporter is pre-validating in detecting extensive DNA damage and intense oxidative stress. Unlike widely-used fluorescent and biolumines-cent biosensors, the visual pigment-based biosensor can become a novel, low-cost, mini-equipment, and high -throughput colorimetric device for the toxicity assessment of chemicals. However, combining multiple im-provements can further improve the biosensing performance in future studies.