Broad-specificity monoclonal antibody against neonicotinoid insecticides via a multi-immunogen strategy and development of a highly sensitive GNP-based multi-residue immunoassay in ginseng and tomato

被引：8

作者：

Zhang, Jing ^{[1
]}

Ruan, Haonan ^{[1
]}

Wang, Yunyun ^{[1
]}

Wang, Yudan ^{[1
]}

Ke, Tongwei ^{[1
]}

Guo, Mengyue ^{[1
]}

Tian, Jiao ^{[1
]}

Huang, Ying ^{[1
]}

Luo, Jiaoyang ^{[1
]}

Yang, Meihua ^{[1
]}

机构：

[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Minist Educ, Key Lab Bioact Subst & Resources Utilizat Chinese, Beijing 100193, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 420卷

基金：

中国国家自然科学基金; 北京市自然科学基金;

关键词：

Neonicotinoid insecticides; Multi-immunogen strategy; Broad-specificity monoclonal antibody; Molecular docking; Gold nanoparticle-based immunochromato; graphic assay; LINKED-IMMUNOSORBENT-ASSAY; LIQUID-CHROMATOGRAPHY; MICROCYSTINS; SAMPLES;

D O I：

10.1016/j.foodchem.2023.136115

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Neonicotinoid insecticides (NNIs) are extensively used across the agricultural products and foods. In order to meet the rapid detection requirements, a novel broad-specificity monoclonal antibody against NNIs was developed for the first time using a multi-immunogen strategy. The antibody's high affinity and its ability to bind target molecules were verified by ic-ELISA. Furthermore, molecular docking was used to evaluate the pivotal forces affecting binding affinity and to determine binding sites. Subsequently, a highly sensitive gold nanoparticle-based immunochromatographic assay was established for the rapid detection of eight NNIs and the IC50 values were 0.03-1.61 ng/mL. The limits of detection for ginseng and tomato ranged from 0.76 to 30.19 mu g/ kg and 0.87 to 31.57 mu g/kg, respectively. The spiked recovery ranged from 72.04% to 120.74%, and the coefficient of variation were less than 9.0%. This study provides a new direction for the development of multiple NNIs residue immunoassays.

引用

页数：11