In-Situ Anaerobic Heating of Human Bones Probed by Neutron Diffraction

被引:1
|
作者
Festa, Giulia [1 ,2 ]
Mamede, Adriana P. [3 ]
Goncalves, David [4 ,5 ,6 ]
Cunha, Eugenia [4 ]
Kockelmann, Winfried [7 ]
Parker, Stewart F. [7 ]
de Carvalho, Luis A. E. Batista [3 ]
Marques, Maria Paula M. [3 ,8 ]
机构
[1] CREF Museo Stor Fis, I-00184 Rome, Italy
[2] Ctr Studi & Ric Enrico Fermi, I-00184 Rome, Italy
[3] Univ Coimbra, Dept Chem, Mol Phys Chem R&D Unit, P-3004535 Coimbra, Portugal
[4] Univ Coimbra, Ctr Funct Ecol, Dept Life Sci, Lab Forens Anthropol, P-3000456 Coimbra, Portugal
[5] Univ Coimbra, Res Ctr Anthropol & Hlth CIAS, P-3000456 Coimbra, Portugal
[6] Directorate Gen Cultural Heritage LARC CIBIO InBIO, Archaeosci Lab, P-1300418 Lisbon, Portugal
[7] STFC Rutherford Appleton Lab, ISIS Pulsed Neutron & Muon Source, Didcot OX11 0QX, England
[8] Univ Coimbra, Dept Life Sci, P-3000456 Coimbra, Portugal
关键词
Anaerobic conditions - Anaerobics - Bone matrix - Cristallinity - Domain size - Human bones - Human femora - Neutron diffraction studies - Neutron scattering techniques - Structural reorganization;
D O I
10.1021/acs.analchem.2c04721
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The first neutron diffraction study of in-situ anaerobic burning of human bones is reported, aiming at an interpretation of heat-induced changes in bone, which were previously detected by vibrational spectroscopy, including inelastic neutron scattering techniques. Structural and crystallinity variations were monitored in samples of the human femur and tibia, as well as a reference hydroxyapatite, upon heating under anaerobic conditions. Information on the structural reorganization of the bone matrix as a function of temperature, from room temperature to 1000 degrees C, was achieved. Noticeable crystallographic and domain size variations, together with O-H bond lengths and background variations, were detected. Above 700 degrees C, the inorganic bone matrix became highly symmetric, devoid of carbonates and organic constituents, while for the lower temperature range (<700 degrees C), a considerably lower crystallinity was observed. The present pilot study is expected to contribute to a better understanding of the heat-prompted changes in bone, which can be taken as biomarkers of the burning temperature. This information is paramount for bone analysis in forensic science as well as in archeology and may also have useful applications in other biomaterial studies.
引用
收藏
页码:2469 / 2477
页数:9
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