Highly Deformable Double-Sided Neural Probe with All-in-One Electrode System for Real-Time In Vivo Detection of Dopamine for Parkinson's Disease

被引：7

作者：

Jung, Han Hee ^{[1
,2
]}

Ha, Jeongdae ^{[1
]}

Park, Jeongrak ^{[2
,3
]}

Kang, Seongtak ^{[4
]}

Kim, Jinmo ^{[2
,5
]}

Jung, Han Na ^{[6
]}

Kim, Samhwan ^{[2
]}

Yea, Junwoo ^{[1
]}

Lee, Hyeokjun ^{[1
,2
]}

Oh, Saehyuck ^{[1
]}

Jekal, Janghwan ^{[1
]}

Song, Soojeong ^{[1
]}

Son, Jieun ^{[1
]}

Yu, Tae Sang ^{[1
]}

Lee, Youngjeon ^{[7
]}

Won, Jinyoung ^{[7
]}

Lim, Kyung Seob ^{[8
]}

Lee, Yoon Kyeung ^{[9
]}

Keum, Hohyun ^{[10
]}

Lee, Taeyoon ^{[11
]}

Song, Young Min ^{[12
]}

Jeong, Jae-Woong ^{[13
]}

Rah, Jong-Cheol ^{[2
,14
]}

Choi, Ji-Woong ^{[2
,5
,14
]}

Xu, Sheng ^{[15
]}

Oh, Yong-Seok ^{[2
,3
,5
,14
]}

Jang, Kyung-In ^{[1
,2
,5
,14
,16
,17
,18
]}

机构：

[1] Daegu Gyeongbuk Inst Sci & Technol DGIST, Dept Robot & Mechatron Engn, Daegu 42988, South Korea

[2] Daegu Gyeongbuk Inst Sci & Technol DGIST, Brain Engn Convergence Res Ctr, Daegu 42988, South Korea

[3] Daegu Gyeongbuk Inst Sci & Technol DGIST, Dept Brain Sci, Daegu 42988, South Korea

[4] Univ Calif San Diego, Dept Neurol, La Jolla, CA 92093 USA

[5] Daegu Gyeongbuk Inst Sci & Technol DGIST, Dept Elect Engn & Comp Sci, Daegu 42988, South Korea

[6] Seoul Natl Univ, Grad Sch Convergence Sci & Technol, Dept Appl Bioengn, Seoul 08826, South Korea

[7] Korea Res Inst Biosci & Biotechnol KRIBB, Natl Primate Res Ctr, Daejeon 28116, South Korea

[8] Korea Res Inst Biosci & Biotechnol KRIBB, Futurist Anim Resource & Res Ctr, Daejeon 28116, South Korea

[9] Jeonbuk Natl Univ, Div Adv Mat Engn, Jeonju 54896, Jeonbuk, South Korea

[10] Korea Inst Ind Technol, Digital Hlth Care R&D Dept, Cheonan 31056, Chungnam, South Korea

[11] Yonsei Univ, Sch Elect & Elect Engn, Seoul 03722, South Korea

[12] Gwangju Inst Sci & Technol, Sch Elect Engn & Comp Sci, Gwangju 61005, South Korea

[13] Korea Adv Inst Sci & Technol, Sch Elect Engn, Daejeon 305701, South Korea

[14] Korea Brain Res Inst, Daegu 41062, South Korea

[15] Univ Calif San Diego, Dept Nanoengn, La Jolla, CA 92093 USA

[16] Daegu Gyeongbuk Inst Sci & Technol DGIST, Artificial Intelligence Major Dept Interdisciplin, Daegu 42988, South Korea

[17] Daegu Gyeongbuk Inst Sci & Technol DGIST, Inst Next Generat Semicond Convergence Technol, Daegu 42988, South Korea

[18] ENSIDE Corp, Daegu 42988, South Korea

来源：

ADVANCED FUNCTIONAL MATERIALS | 2024年 / 34卷 / 04期

基金：

新加坡国家研究基金会;

关键词：

dopamine; electrochemical biosensors; neural probes; Parkinson's disease; soft mechanics; LIQUID-CHROMATOGRAPHY; MICROELECTRODE ARRAY; POLYMER; IMMOBILIZATION; MICRODIALYSIS; RELEASE; FILM;

D O I：

10.1002/adfm.202311436

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Precise monitoring of neurotransmitters, such as dopamine (DA), is critical for understanding brain function and treating neurological disorders since dysregulation of DA implicates in a range of disorders, including Parkinson's disease (PD), schizophrenia, and addiction. This study proposes a multi-deformable double-sided (MDD) DA-sensing probe with the three-electrode system in all-in-one form for reliable real-time monitoring of DA dynamics by integrating working, reference, and counter electrodes on a single probe. The proposed probe achieves high DA sensitivity and selectivity in virtue of enzyme immobilization on the 3D nanostructures grown on working electrode. Also, the serpentine design is employed for the electrodes to withstand in various deformations by achieving high stretchability and manage the stress induced on the probe. Experimental and computational analysis demonstrates an effective reduction in induced-stress on the electrodes. The MDD DA-sensing probe is implanted into the brain with success to enable real-time, in vivo monitoring of DA levels in rodents. Furthermore, DA dynamic changes are monitored before and after treatment with L-DOPA in hemi-PD mice. This extremely deformable implantable probe has the potential for use in the study and treatment of neurodegenerative diseases, providing reliable monitoring of DA dynamics with minimal damage to brain tissue.

引用

页数：13

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[1] Nanoscale size effects on the mechanical properties of platinum thin films and cross-sectional grain morphology [J].