Collagen-Laponite Nanoclay Hydrogels for Tumor Spheroid Growth

被引：7

作者：

Alaman-Diez, Pilar ^{[1
,2
]}

Borau, Carlos ^{[1
,2
]}

Guerrero, Pedro Enrique ^{[1
,2
]}

Amaveda, Hippolyte ^{[3
,4
]}

Mora, Mario ^{[3
,4
]}

Fraile, Jose Maria ^{[5
]}

Garcia-Gareta, Elena ^{[1
,2
,6
]}

Garcia-Aznar, Jose Manuel ^{[1
,2
]}

Perez, Maria Angeles ^{[1
,2
]}

机构：

[1] Univ Zaragoza, Aragon Inst Engn Res I3A, Multiscale Mech & Biol Engn, Zaragoza 50018, Spain

[2] Univ Zaragoza, Aragon Inst Healthcare Res IIS Aragon, Dept Mech Engn, Zaragoza 50018, Spain

[3] Univ Zaragoza, Aragon Inst Nanosci & Mat INMA, Zaragoza 50018, Aragon, Spain

[4] CSIC, Zaragoza 50018, Spain

[5] Univ Zaragoza, Inst Chem Synth & Homogeneous Catalysis ISQCH, Zaragoza 50009, Aragon, Spain

[6] UCL, UCL Eastman Dent Inst, Div Biomat & Tissue Engn, London WC1E 6BT, England

来源：

BIOMACROMOLECULES | 2023年 / 24卷 / 06期

基金：

欧洲研究理事会;

关键词：

CLAY; 3D; FABRICATION; DELIVERY;

D O I：

10.1021/acs.biomac.3c00257

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The extracellular matrix (ECM) plays an important regulatoryrolein the development and progression of tumoral tissue. Its functionsand properties are crucial in determining tumor cell behavior suchas invasion, migration, and malignancy development. Our study exploresthe role of collagen type I in cancer development and spread usingengineered tumor models like multicellular spheroids grown in collagen-basedhydrogels to simulate early tumor formation. We employ microfluidictechniques to test the hypothesis that (i) adding Laponite nanoclayto collagen hydrogels modifies mechanical and rheological propertiesand (ii) changing the stiffness of the collagen microenvironment affectstumor spheroid growth. Our findings support our theories and suggestthe use of ECM components and engineered tumor models in cancer research,offering a biocompatible and biomimetic method to tailor the mechanicalproperties of conventional collagen hydrogels.

引用

页码：2879 / 2891

页数：13

共 65 条

[1] Integrative In Situ Photodynamic Therapy-Induced Cell Death Measurement of 3D-Bioprinted MCF-7 Tumor Spheroids [J].