DNA Barcoding of Pyrrhulina australis (Characiformes: Lebiasinidae) reveals unexpected cryptic diversity in the group

被引:1
作者
de Souza, Taina Barbosa [1 ]
Ferreira, Daniela Cristina [2 ]
da Silva, Hugmar Pains [3 ]
Netto-Ferreira, Andre Luiz [4 ]
Venere, Paulo Cesar [2 ]
机构
[1] Univ Fed Mato Grosso, Programa Pos Grad Ecol & Conservacao Biodiversida, Ave Fernando Correa da Costa 2367, BR-78060900 Cuiaba, MT, Brazil
[2] Univ Fed Mato Grosso, Inst Biociencias, Lab Genet & Citogenet Anim, Av Fernando Correa da Costa 2367, BR-78060900 Cuiaba, MT, Brazil
[3] Univ Estado Mato Grosso, Fac Ciencias Agr Biol Engn & Saude, BR-78301532 Tangara Da Serra, MT, Brazil
[4] Univ Fed Rio Grande do Sul, Inst Biociencias, Dept Zool, Lab Ictiol, Ave Bento Goncalves 9500, BR-91501970 Porto Alegre, RS, Brazil
关键词
Cryptic species; Freshwater fish; Molecular identification; Small fishes; Species delimitation methods; BRAZILIAN SHIELD; FISH DIVERSITY; RIVER-BASIN; EVOLUTIONARY; INVENTORY; FISHERIES; HABITATS; SERRA;
D O I
10.1590/1982-0224-2023-0037
中图分类号
Q95 [动物学];
学科分类号
071002 ;
摘要
The family Lebiasinidae includes a number of miniature and medium-sized fish species that are endemic to the Neotropical region. Pyrrhulina is the second most speciose lebiasinid genus and it is also the one with the most taxonomic uncertainties. In this context, the present study focused on the Pyrrhulina morphospecies found in a number of different drainage basins in South America to test the alternative proposals on the arrangement of the taxonomic units found within what is assumed to be a single nominal species, Pyrrhulina australis, based on a DNA Barcoding approach. The results of the analyses indicate that Pyrrhulina australis is a species complex, with intraspecific (within-group) genetic distances of up to 3.74%, well above the Optimal Threshold of 1.79% defined in the present study. The species delimitation analyses revealed a surprising level of diversification among the morphospecies evaluated, in particular, in the clade that encompasses Pyrrhulina australis (from the Paraguay River) + P. cf. rachoviana (Lower Parana River), P. aff. australis I (Araguaia River)/II (Paraguay River)/III (Upper Parana River)/IV (Guapore River), and P. marilynae (Teles Pires River), which were arranged in six distinct evolutionary lineages that align with the geographical distribution of the respective drainage basins.
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页数:22
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