Leaf Biochemical and Kernel Metabolite Profiles as Potential Biomarkers of Water Deficit in Walnut (Juglans regia L.) cv. Chandler

被引:0
作者
Calvo, Franco E. [1 ,2 ]
Silvente, Sonia T. [3 ]
Trentacoste, Eduardo R. [4 ]
机构
[1] Consejo Nacl Invest Cient & Tecn CONICET, RA-1425 Buenos Aires, Argentina
[2] Univ Nacl Chilecito UNdeC, Inst Agr Sostenible Oasis IASO, RA-5360 Chilecito, Argentina
[3] Univ Nacl Chilecito UNdeC, Inst Ambiente Montana & Reg Aridas IAMRA, RA-5360 Chilecito, Argentina
[4] Inst Nacl Tecnol Agr, Estn Expt Agr Consulta, RA-5567 Mendoza, Argentina
关键词
chandler; metabolomics; water stress; phenology; deficit irrigation; SOLUBLE SUGARS; WHITE CLOVER; PROLINE; ACCUMULATION; METABOLOMICS; TOLERANCE; CHEMISTRY; DROUGHT; GROWTH; PLANT;
D O I
10.3390/su151813472
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Walnut cultivation is expanding into regions where water availability for irrigation is lower than crop evapotranspiration. However, information regarding the responses and adaptations of walnut trees to water deficit remains scarce. In this study, we applied three irrigation levels, 100%, 75%, and 50% of crop evapotranspiration (referred to as T100, T75, and T50, respectively), to Chandler walnut trees over two consecutive seasons. During the second season, we evaluated leaf water-deficit biomarkers, including proline, malondialdehyde, soluble sugars, phenols, and flavonoids, using targeted spectrophotometry. Despite not finding significant differences in biomarker concentrations among the irrigation regimes, we observed variations between different collection times (sprouting, endocarp hardening, and maturity). Furthermore, we assessed the kernel metabolome using untargeted gas chromatography-mass spectrometry, profiling seventy-one metabolites across all samples. Notably, forty-one of these metabolites were identified as members of distinct groups, comprising carbohydrates (n = 11), fatty acids (n = 11), organic acids (n = 9), and amino acids (n = 5). Linear mixed models showed no significant differences between the irrigation regimes. However, in the T50 treatment, multivariate analysis (PCA) revealed a higher concentration of osmotic adjustment metabolites, which are potentially associated with protecting oil biosynthesis under high-temperature and water deficit conditions.
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页数:14
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