Peptide-based probe for colorimetric and fluorescent detection of Cu2+and S2-in environmental and biological systems

被引:50
作者
An, Yong [1 ]
Li, Linyu [1 ]
Li, Lepeng [1 ]
Sun, Yongqiang [1 ]
Li, Bo [1 ,2 ]
Wang, Peng [3 ]
机构
[1] Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou 730000, Gansu, Peoples R China
[2] Gansu Prov Hosp, Dept Musculoskeletal Tumor, Lanzhou 730000, Gansu, Peoples R China
[3] China West Normal Univ, Coll Chem & Chem Engn, Chem Synth & Pollut Control Key Lab Sichuan Prov, Nanchong 637009, Peoples R China
基金
中国国家自然科学基金;
关键词
Copper ions; Hydrogen sulfide; Colorimetry; On-site visualization; Peptide-based; COPPER; IONS; CHEMOSENSOR; DEATH;
D O I
10.1016/j.jhazmat.2023.133192
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Pollution caused by Copper and hydrogen sulfide pollution has severe adverse effects on the environment and organisms. Real-time, fast and accurate monitoring of Cu2+ and S2-faces serious challenges. In this study, we designed a novel biosensor and synthesized it by mimicking the structure of the main Cu(II)-binding site on bovine serum albumin. As a peptide-based sensor, FGGH (FITC-Gly-Gly-His-NH2) can perform the sequential detection of Cu2+ and S2- by fluorescence and colorimetry. The high water solubility and selectivity make it suitable for monitoring Cu2+ and S2- in environmental water samples with high sensitivity; its limit of detection (LOD) is as low as 1.42 nM for Cu2+ and 22.2 nM for S2-. The paper-based sensing platform of this probe was found to be a promising tool for the on-site visualization of real-time quantitative analysis of Cu2+ and S2- due to its rapid response and recyclable detection characteristics. Additionally, FGGH was successfully used to image Cu2+ and S2- in living cells and zebrafish models with adequate fluorescence stability and low cytotoxicity, providing the first visual evidence of the effect of the interactions between Cu2+ and S2- on the redox homeostasis of organisms.
引用
收藏
页数:12
相关论文
共 50 条
[41]   An innovative fluorescent probe based on dicyanoisoflurone derivatives for differential detection of Hg2+and Cu2+and its applications in bacteria, cell imaging and food analysis [J].
Yao, Yixuan ;
Ma, Jinli ;
Xing, Shuo ;
Zeng, Fudong ;
Wu, Liangqiang ;
Li, Yapeng ;
Du, Jianshi ;
Yang, Qingbiao ;
Li, Yaoxian .
ANALYTICA CHIMICA ACTA, 2024, 1292
[42]   A dual functional fluorescent probe based on naphthalimide for detecting Cu2+and pH and its applications [J].
Zhang, Chenglu ;
Wang, Yiming ;
Li, Xiangling ;
Nie, Shiru ;
Liu, Chang ;
Zhang, Yang ;
Guo, Jinghao ;
Liu, Cui .
INORGANICA CHIMICA ACTA, 2023, 554
[43]   Dual Channel Rhodamine Based Colorimetric Fluorescent Probe for Cu2+ in Living Cells [J].
Jiang Li ;
Zhang Bo ;
Sun Jian ;
Yin Gui ;
Shang Tong-Ming ;
Wang Ling ;
Wang Rui-Yong .
CHINESE JOURNAL OF INORGANIC CHEMISTRY, 2010, 26 (10) :1750-1755
[44]   triphenylamine-isophorone-based "off-on" fluorescent and colorimetric probe for Cu2+ [J].
Li, Wei ;
Zhang, Ying ;
Gan, Xiaoping ;
Yang, Mingdi ;
Mie, Bin ;
Fang, Min ;
Zhang, Qiyan ;
Yu, Jianhua ;
Wu, Jieying ;
Tian, Yupeng ;
Zhou, Hongping .
SENSORS AND ACTUATORS B-CHEMICAL, 2015, 206 :640-646
[45]   A highly selective colorimetric and fluorescent probe for Cu2+ based diarylethene with a diaminomaleonitrile unit [J].
Liu, Xutang ;
Liu, Hongliang ;
Tang, Xugang ;
Liu, Gang ;
Pu, Shouzhi .
TETRAHEDRON, 2021, 78
[46]   A dual-signals fluorometric and colorimetric peptide-based probe for Cu(II) and glyphosate detection and its application for bioimaging and water testing [J].
Li, Shiyang ;
Pu, Chunmei ;
Cao, Xinlin ;
Zheng, Maoyue ;
Deng, Weiliang ;
Wang, Peng ;
Wu, Jiang .
SCIENCE OF THE TOTAL ENVIRONMENT, 2024, 945
[47]   An ?AIE plus ESIPT? characteristic fluorescent probe for relay recognition of Cu2+and H2S and its application in food samples and cell imaging [J].
Pan, Yongxin ;
Li, Ying ;
Sun, Xiaofei ;
Tang, Lijun ;
Yan, Xiaomei .
DYES AND PIGMENTS, 2023, 210
[48]   Synthesis and application of fluorescent probes for Cu2+and H2S detection based on Rhodol's "on-off-on" mechanism [J].
Tang, Jiefeng ;
Chen, Xiangjun ;
Wang, Xiaoqian ;
Zhang, Shuntao ;
Wang, Juan ;
Cheng, Chunru .
JOURNAL OF MOLECULAR STRUCTURE, 2024, 1318
[49]   Irreversible coumarin based fluorescent probe for selective detection of Cu2+ in living cells [J].
Ahmed, Nadeem ;
Zareen, Wajeeha ;
Zhang, Di ;
Yang, Xiaopeng ;
Ye, Yong .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 264
[50]   An efficient tetrahydroquinazolin-2-amine derivative-grafted cellulose fluorescent probe for detection of Cu2+and Zn2+ [J].
Meng, Zhiyuan ;
Zhao, Fei ;
Wang, Zhonglong ;
Yang, Yiqin ;
Wang, Shifa .
CARBOHYDRATE POLYMERS, 2023, 303