Viable but nonculturable (VBNC) state, an underestimated and controversial microbial survival strategy

被引：77
作者：
Liu, Junyan [1 ,2 ]
Yang, Liang [3 ]
Kjellerup, Birthe Veno [4 ]
Xu, Zhenbo [5 ,6 ]
机构：
[1] Zhongkai Univ Agr & Engn, Acad Con orary Agr Engn Innovat, Coll Light Ind & Food Sci, Guangdong Prov Key Lab Lingnan Specialty Food Sci, Guangzhou 510225, Peoples R China
[2] Key Lab Green Proc & Intelligent Mfg Lingnan Speci, Minist Agr, Guangzhou 510225, Peoples R China
[3] Southern Univ Sci & Technol, Sch Med, Shenzhen 518055, Peoples R China
[4] Univ Maryland, Dept Civil & Environm Engn, College Pk, MD 20742 USA
[5] South China Univ Technol, Engn Res Ctr Starch & Vegetable Prot Proc, Guangdong Prov Key Lab Green Proc Nat Prod & Prod, Sch Food Sci & Engn,Minist Educ, Guangzhou 510640, Peoples R China
[6] Shantou Univ, Affiliated Hosp 2, Med Coll, Dept Lab Med, Shantou, Peoples R China
来源：
TRENDS IN MICROBIOLOGY | 2023年 / 31卷 / 10期
基金：
中国国家自然科学基金;
关键词：
NON-CULTURABLE STATE; ESCHERICHIA-COLI; VIBRIO-CHOLERAE; SPOILAGE CAPABILITY; VIABILITY; RESUSCITATION; DISINFECTION; BIOFILMS; CELLS; SPP;
D O I：
10.1016/j.tim.2023.04.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
As a unique microbial response to adverse circumstances, the viable but nonculturable (VBNC) state is characterized by the loss of culturability of microbial cells on/in nutrient media that normally support their growth, while maintaining metabolic activity. These cells can resuscitate to a culturable state under suitable conditions. Given the intrinsic importance of the VBNC state and recent debates surrounding it, there is a need to redefine and standardize the term, and to address essential questions such as 'How to differentiate VBNC from other similar terms?' and 'How can VBNC cells be standardly and accurately determined?'. This opinion piece aims at contributing to an improved understanding of the VBNC state and promoting its proper handling as an underestimated and controversial microbial survival strategy.
引用
收藏
页码：1013 / 1023
页数：11
相关论文
共 90 条
  • [21] Fakruddin Md, 2013, ISRN Microbiol, V2013, P703813, DOI 10.1155/2013/703813
  • [22] Evidence of Helicobacter spp. in freshwaters from Roraima Tepui, Guayana Shield, South America
    Fernandez-Delgado, Milagro
    Giarrizzo, Juan G.
    Garcia-Amado, Maria A.
    Contreras, Monica
    Salazar, Victor
    Barton, Hazel
    Suarez, Paula
    [J]. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2016, 109 (04): : 529 - 542
  • [23] Microbial detection and identification methods: Bench top assays to omics approaches
    Ferone, Mariateresa
    Gowen, Aoife
    Fanning, Seamus
    Scannell, Amalia G. M.
    [J]. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, 2020, 19 (06) : 3106 - 3129
  • [24] Role of food sanitising treatments in inducing the 'viable but nonculturable' state of microorganisms
    Ferro, Sergio
    Amorico, Tony
    Deo, Permal
    [J]. FOOD CONTROL, 2018, 91 : 321 - 329
  • [25] Monitoring of bacterial load in terms of culturable and non-culturable cells on new materials placed in a delicatessen serve over counter
    Firmesse, Olivier
    Morelli, Elisabeth
    Vann, Sokchea
    Carpentier, Brigitte
    [J]. INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 159 (03) : 179 - 185
  • [26] Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels
    Garcia-Hernandez, Jorge
    Hernandez, Manuel
    Moreno, Yolanda
    [J]. FOODS, 2021, 10 (07)
  • [27] Development of a Direct and Rapid Detection Method for Viable but Non-culturable State of Pediococcus acidilactici
    Guan, Yu
    Wang, Kan
    Zeng, Yang
    Ye, Yanrui
    Chen, Ling
    Huang, Tengyi
    [J]. FRONTIERS IN MICROBIOLOGY, 2021, 12
  • [28] EmPis-1L, an Effective Antimicrobial Peptide Against the Antibiotic-Resistant VBNC State Cells of Pathogenic Bacteria
    Hu, Bochao
    Pan, Yanqiu
    Li, Zhenping
    Yuan, Weiming
    Deng, Li
    [J]. PROBIOTICS AND ANTIMICROBIAL PROTEINS, 2019, 11 (02) : 667 - 675
  • [29] Rapid and Simultaneous Detection of Five, Viable, Foodborne Pathogenic Bacteria by Photoinduced PMAxx-Coupled Multiplex PCR in Fresh Juice
    Huang, Tiantian
    Shi, Yaoqiang
    Zhang, Jinyang
    Han, Qinqin
    Xia, Xue-shan
    Zhang, A-Mei
    Song, Yuzhu
    [J]. FOODBORNE PATHOGENS AND DISEASE, 2021, 18 (09) : 640 - 646
  • [30] Overview of VBNC, a survival strategy for microorganisms
    Izgordu, Ozge Kaygusuz
    Darcan, Cihan
    Kariptas, Ergin
    [J]. 3 BIOTECH, 2022, 12 (11)
123456789