Untargeted metabolomics reveals the effect of rearing systems on bone quality parameters in chickens

被引:2
作者
Li, Dongfeng [1 ]
Wu, Yongfu [1 ]
Shi, Kai [1 ]
Shao, Minghui [1 ]
Duan, Ying [1 ]
Yu, Minli [1 ]
Feng, Chungang [1 ]
机构
[1] Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing, Jiangsu, Peoples R China
基金
国家重点研发计划;
关键词
chickens; bone quality parameters; rearing systems; metabolomics; LC-MS/MS; MINERAL DENSITY; LAYING HENS; GROWTH; ASSOCIATION; TIBIA; BROILERS; OSTEOPOROSIS; METABOLISM; MORPHOLOGY; STRENGTH;
D O I
10.3389/fgene.2022.1071562
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The objective of this study was to investigate the effects of rearing systems on the bone quality parameters in chickens using a metabolomics strategy. A total of 419 male one-day-old chicks were randomly allocated to two groups, a floor rearing group (FRG, n = 173) and a cage rearing group (CRG, n = 246). At 6, 8, 10, and 12 weeks of age, all chickens were radiographed by a digital X-ray machine, and body weight was recorded. At 12 weeks of age, 12 birds were selected from each group to obtain tibia and femur, and bone quality parameters of bone mineral density (BMD), mineral content (BMC), breaking strength (BBS), stiffness, Young's modulus (YM), ash content, calcium content, and phosphorus content were determined. An untargeted metabolomics assay was performed to identify changes in the serum metabolic profile (n = 8 birds/group). The results showed that cage-reared chickens had wider tibiae and greater body weight compared with floor-reared chickens. There were no significant differences in BMC or BBS between the two groups (p > 0.05), but BMD, ash content, calcium content, and phosphorus content of the tibia and femur of FRG were significantly higher than those of CRG (p < 0.05). Greater stiffness and YM of the femur were also observed in birds raised in the FRG compared with those raised in the CRG (p < 0.05). Taken together, the results suggest that rearing systems affected bone quality parameters. Furthermore, 148 and 149 differential metabolites were identified in positive and negative ion modes by LC-MS/MS analysis, among which 257 metabolites were significantly correlated with 16 bone quality parameters, including leucine, myristoleic acid, glycocholic acid, and N-phenylacetamide. KEGG analysis indicated that 15 metabolic pathways, including six pathways of amino acid metabolism, two pathways of lipid metabolism, and two pathways of carbohydrate metabolism, were responsible for bone quality. Overall, the present study demonstrated the effect of rearing systems on bone quality parameters, and identified several metabolites and metabolic pathways associated with bone quality parameters.
引用
收藏
页数:11
相关论文
共 50 条
  • [41] Genome-wide association study of growth curve parameters reveals novel genomic regions and candidate genes associated with metatarsal bone traits in chickens
    Wang, S. Z.
    Wang, M. D.
    Wang, J. Y.
    Yuan, M.
    Li, Y. D.
    Luo, P. T.
    Xiao, F.
    Li, H.
    ANIMAL, 2024, 18 (04)
  • [42] Effect of Disinfectants on Physicochemical Parameters of Litter, Microbiological Quality of Poultry House Air, Health Status and Performance of Broiler Chickens
    Mituniewicz, T.
    Sowinska, J.
    Wojcik, A.
    Iwanczuk-Czernik, K.
    Witkowska, D.
    Banas, J.
    POLISH JOURNAL OF ENVIRONMENTAL STUDIES, 2008, 17 (05): : 745 - 750
  • [43] Effect of Cage and Floor Housing Systems on Muscle Fiber Characteristics, Carcass Characteristics, and Meat Quality of Slow-Growing Meat-Type Chickens
    Sun, Yanyan
    Liu, Chen
    Li, Yunlei
    Li, Dongli
    Shi, Lei
    Chen, Jilan
    AGRICULTURE-BASEL, 2023, 13 (02):
  • [44] Effect of Pre-Treating Dietary Green Seaweed with Proteolytic and Fibrolytic Enzymes on Physiological and Meat Quality Parameters of Broiler Chickens
    Ben Matshogo, Tumisang
    Mnisi, Caven Mguvane
    Mlambo, Victor
    FOODS, 2021, 10 (08)
  • [45] Effect of botanical composition of free-range areas on production results and selected meat quality parameters of organic Yellowleg Partridge chickens
    Sosnowka-Czajka, Ewa
    Skomorucha, Iwona
    Radkowska, Iwona
    ANNALS OF ANIMAL SCIENCE, 2023, 23 (02) : 571 - 585
  • [46] Effect of 25-Hydroxycholecalciferol with Different Vitamin D3 Levels in the Hens Diet in the Rearing Period on Growth Performance, Bone Quality, Egg Production, and Eggshell Quality
    Li, Dongdong
    Zhang, Keying
    Bai, Shiping
    Wang, Jianping
    Zeng, Qiufeng
    Peng, Huanwei
    Su, Zuowei
    Xuan, Yue
    Qi, Sharina
    Ding, Xuemei
    AGRICULTURE-BASEL, 2021, 11 (08):
  • [47] Dynamic modeling of recirculating aquaculture systems: Effect of management strategies and water quality parameters on fish performance
    Kamali, Sara
    Ward, Valerie C. A.
    Ricardez-Sandoval, Luis
    AQUACULTURAL ENGINEERING, 2022, 99
  • [48] Effect of pre-treating dietary green seaweed with fibrolytic enzymes on growth performance, blood indices, and meat quality parameters of Cobb 500 broiler chickens
    Matshogo, T. B.
    Mlambo, V.
    Mnisi, C. M.
    Manyeula, F.
    LIVESTOCK SCIENCE, 2021, 251
  • [49] Effect of supplementing microbial phytase in diets containing graded concentrations of calcium on performance, shell quality and bone mineral parameters in WL layers
    Rao, S. V. Rama
    Raju, M. V. L. N.
    Panda, A. K.
    Murthi, O. Krishna
    ANIMAL FEED SCIENCE AND TECHNOLOGY, 2014, 193 : 102 - 110
  • [50] Effect of Using Vitamin C Supplementation on Performance, Blood Parameters, Carcass Characteristics and Meat Quality of Broiler Chickens Under Heat Stress Condition: A Meta-Analysis
    Tavakolinasab, Fatemeh
    Hashemi, Mazaher
    JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, 2025,