Analysis and validation of overall N-glycan conformation in Privateer

被引：9
|
作者：
Dialpuri, Jordan S. [1 ]
Bagdonas, Haroldas [1 ]
Atanasova, Mihaela [1 ]
Schofield, Lucy C. [1 ]
Hekkelman, Maarten L. [2 ,3 ]
Joosten, Robbie P. [2 ,3 ]
Agirre, Jon [1 ]
机构：
[1] Univ York, Dept Chem, York Struct Biol Lab, York YO10 5DD, N Yorkshire, England
[2] Netherlands Canc Inst, Oncode Inst, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands
[3] Netherlands Canc Inst, Div Biochem, Plesmanlaan 121, NL-1066 CX Amsterdam, Netherlands
来源：
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2023年 / 79卷
基金：
英国工程与自然科学研究理事会; 英国生物技术与生命科学研究理事会;
关键词：
glycobiology; validation; Privateer; N-glycans; torsion angles; CARBOHYDRATE STRUCTURE; CRYSTAL-STRUCTURE; PROTEIN; COMPLEX; GLYCOSYLATION;
D O I：
10.1107/S2059798323003510
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The oligosaccharides in N-glycosylation provide key structural and functional contributions to a glycoprotein. These contributions are dependent on the composition and overall conformation of the glycans. The Privateer software allows structural biologists to evaluate and improve the atomic structures of carbohydrates, including N-glycans; this software has recently been extended to check glycan composition through the use of glycomics data. Here, a broadening of the scope of the software to analyse and validate the overall conformation of N-glycans is presented, focusing on a newly compiled set of glycosidic linkage torsional preferences harvested from a curated set of glycoprotein models.
引用
收藏
页码：462 / 472
页数：11
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