Screening for Metallo-Beta-Lactamases Using Non-Carbapenem Agents: Effective Detection of MBL-Producing Enterobacterales and Differentiation of Carbapenem-Resistant Enterobacterales

被引：1

作者：

Takei, Kentarou ^{[1
]}

Kanamori, Hajime ^{[1
]}

Nakayama, Asami ^{[2
]}

Chiba, Mikiko ^{[2
]}

Takei, Yumiko ^{[1
]}

Seike, Issei ^{[1
]}

Kitamura, Chiho ^{[1
]}

Baba, Hiroaki ^{[1
]}

Oshima, Kengo ^{[1
]}

Tokuda, Koichi ^{[1
]}

机构：

[1] Tohoku Univ, Dept Infect Dis, Internal Med, Grad Sch Med, Sendai 9808574, Japan

[2] Tohoku Univ Hosp, Dept Lab Med, Sendai 9808574, Japan

来源：

ANTIBIOTICS-BASEL | 2023年 / 12卷 / 07期

基金：

日本学术振兴会;

关键词：

ceftazidime; cefoperazone/sulbactam; carbapenem-resistant Enterobacterales; metallo-beta-lactamase; RISK-FACTORS; EPIDEMIOLOGY; PREVALENCE;

D O I：

10.3390/antibiotics12071146

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Metallo-beta-lactamases (MBLs) are enzymes that break down carbapenem antibiotics, leading to carbapenem-resistant organisms. Carbapenemase-resistant Enterobacterales (CRE) is one of them. Outbreaks of CRE infection can occur in healthcare facilities and lead to increased deaths, illness, and medical costs. This study was conducted to detect MBLs using non-carbapenem agents and exclude MBLs among CRE isolates. A total of 3776 non-duplicate sequential Enterobacterales isolates from a single facility were screened between January 2019 and December 2022 using non-carbapenem agents, ceftazidime and cefoperazone/sulbactam. Positive 153 isolates (4.0%) were further tested using carbapenemase-confirmation tests and verified through polymerase chain reaction (PCR) testing. Fifteen imipenemase (IMP)-type MBL-producing Enterobacterales (0.4%) including one susceptible to carbapenems were identified. Moreover, 160 isolates (4.2%) meeting the criteria for CRE were directly subjected to PCR testing. All fourteen CRE isolates with MBLs identified through PCR testing were found to be the same strains screened using ceftazidime and cefoperazone/sulbactam. Screening using ceftazidime and cefoperazone/sulbactam can effectively detect MBL-producing Enterobacterales strains. This screening method showed comparable results to screening with meropenem, potentially serving as a supplementary approach and contributing to differentiating between MBL- and nonMBL-producing CRE strains. Our findings support these screening methods, particularly in regions where IMP-type MBLs are prevalent.

引用

页数：9