Investigation of mechanistic interactions between Rifampicin and bovine serum albumin in the presence of different surfactants

Rifampicin, is an antibiotic used in the treatment of numerous types of bacterial infections, including tuberculosis, Mycobacterium avium complex, leprosy, and Legionnaires' disease. To explicate the binding interactions of Rifampicin with bovine serum albumin (BSA) at molecular level, in the presence of cationic, anionic, and neutral surfactants, it is urgent to investigate their interactions to accomplish its pharmacokinetic and pharmacodynamics gaps vital for advance development and improvement as a therapeutic drug. Thus, we have investigated the binding interaction of Rifampicin with BSA at physiological pH 7.4 in the presence and absence of surfactants Sodium dodecyl sulfate, Cetyltrimethylammonium bromide, and Tween 80. The multispectroscopic studies of Rifampicin with BSA as investigated by UV-visible, fluorescence, and circular dichroism spectroscopy shown a significant stabilization of BSA in the presence of surfactants; that can help in designing more competent drug formulations from a pharmaceutical point of view.