A Practical Method for Amino Acid Analysis by LC-MS Using Precolumn Derivatization with Urea

被引:5
|
作者
Zhao, Runjin [1 ,2 ]
Huang, Biling [1 ,2 ]
Lu, Gang [1 ]
Fu, Songsen [1 ,2 ]
Ying, Jianxi [1 ,2 ]
Zhao, Yufen [1 ,2 ,3 ,4 ]
机构
[1] Ningbo Univ, Inst Drug Discovery Technol, Ningbo 315211, Peoples R China
[2] Ningbo Univ, Qian Xuesen Collaborat Res Ctr Astrochem & Space L, Ningbo 315211, Peoples R China
[3] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem Biol, Xiamen 361005, Peoples R China
[4] Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China
基金
中国国家自然科学基金;
关键词
amino acids; quantitative analysis; derivatization; urea; LC-MS; PERFORMANCE LIQUID-CHROMATOGRAPHY; REAGENTS; CHLORIDE; IDENTIFICATION;
D O I
10.3390/ijms24087332
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Amino acid (AA) analysis is important in biochemistry, food science, and clinical medicine. However, due to intrinsic limitations, AAs usually require derivatization to improve their separation and determination. Here, we present a liquid chromatography-mass spectrometry (LC-MS) method for the derivatization of AAs using the simple agent urea. The reactions proceed quantitatively under a wide range of conditions without any pretreatment steps. Urea-derivatized products (carbamoyl amino acids) of 20 AAs exhibit better separation on reversed-phase columns and increased response in a UV detector compared to underivatized ones. We applied this approach to AA analysis in complex samples using a cell culture media as a model, and it showed potential for the determination of oligopeptides. This fast, simple, and inexpensive method should be useful for AA analysis in complex samples.
引用
收藏
页数:10
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