Soluble dietary fibres decrease ?-glucosidase inhibition of epigallocatechin gallate through affecting polyphenol-enzyme binding interactions

被引：28

作者：

Li, Jing ^{[1
]}

Zhang, Jifan ^{[1
]}

Yu, Wanyi ^{[1
]}

Gao, Hang ^{[1
]}

Szeto, Ignatius M. Y. ^{[2
]}

Feng, Haotian ^{[3
]}

Liu, Xuebo ^{[1
]}

Wang, Yutang ^{[1
]}

Sun, Lijun ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Food Sci & Engn, Xianyang, Peoples R China

[2] Inner Mongolia Yili Ind Grp Co Ltd, Hohhot, Peoples R China

[3] Inner Mongolia Dairy Technol Res Inst Co Ltd, Hohhot, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 409卷

基金：

中国国家自然科学基金;

关键词：

Soluble dietary fibres; Enzyme inhibition; Epigallocatechin gallate; Digestion; Binding interactions; ALPHA-AMYLASE; TEA POLYPHENOLS; POLYSACCHARIDES; ADSORPTION; DIGESTION; EGCG;

D O I：

10.1016/j.foodchem.2022.135327

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The effects of soluble dietary fibres (SDFs) on alpha-glucosidase inhibition of EGCG were studied. Three arabinox-ylans and polygalacturonic acid (PGA) significantly decreased inhibitory activity of EGCG against alpha-glucosidase, while two beta-glucans hardly affected the inhibition. Although arabinoxylans and PGA weakened the competitive inhibition character of EGCG, they maintained the fluorescence quenching effect of EGCG. Then, arabinoxylans and PGA significantly decreased the particle size and turbidity of EGCG-enzyme complex. These results suggest that there formed SDFs-EGCG-enzyme ternary complexes. The stronger decreasing-effects of arabinoxylans and PGA on alpha-glucosidase inhibition of EGCG than beta-glucans resulted from the stronger non-covalent interactions of arabinoxylans and PGA with EGCG. This is considered to arise from the short-branches of arabinoxylans that provided more opportunity for capturing EGCG, and from the strong polarity of PGA carboxyl that promoted hydrogen bondings with EGCG. Conclusively, SDFs should be considered as an impact factor when evaluating alpha-glucosidase inhibition of dietary polyphenols.

引用

页数：13

共 40 条

[1] FTIR and Raman signatures of wheat grain peripheral tissues [J].

Barron, C. ;

Rouau, X. .

CEREAL CHEMISTRY, 2008, 85 (05) :619-625

[2] Impact of postprandial glycaemia on health and prevention of disease [J].

Blaak, E. E. ;

Antoine, J. -M. ;

Benton, D. ;

Bjorck, I. ;

Bozzetto, L. ;

Brouns, F. ;

Diamant, M. ;

Dye, L. ;

Hulshof, T. ;

Holst, J. J. ;

Lamport, D. J. ;

Laville, M. ;

Lawton, C. L. ;

Meheust, A. ;

Nilson, A. ;

Normand, S. ;

Rivellese, A. A. ;

Theis, S. ;

Torekov, S. S. ;

Vinoy, S. .

OBESITY REVIEWS, 2012, 13 (10) :923-984

[3] The role of wine polysaccharides on salivary protein-tannin interaction: A molecular approach [J].

Brandao, Elsa ;

Silva, Mafalda Santos ;

Garcia-Estevez, Ignacio ;

Williams, Pascale ;

Mateus, Nuno ;

Doco, Thierry ;

de Freitas, Victor ;

Soares, Susana .

CARBOHYDRATE POLYMERS, 2017, 177 :77-85

[4] USE OF DIXON PLOTS TO STUDY ENZYME INHIBITION [J].

BUTTERWORTH, PJ .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 289 (02) :251-253

[5] Number of galloyl moiety and intramolecular bonds in galloyl-based polyphenols affect their interaction with alpha-glucosidase [J].

Cao, Junwei ;

Yan, Shaoqing ;

Xiao, Yao ;

Han, Lin ;

Sun, Lijun ;

Wang, Min .

FOOD CHEMISTRY, 2022, 367

[6] The effect of viscous soluble dietary fiber on nutrient digestion and metabolic responses I: In vitro digestion process [J].

Chen, Maoshen ;

Guo, Liping ;

Nsor-Atindana, John ;

Goff, H. Douglas ;

Zhang, Wenxi ;

Mao, Jian ;

Zhong, Fang .

FOOD HYDROCOLLOIDS, 2020, 107

[7] Cyclodextrin-based metal-organic framework nanoparticles as superior carriers for curcumin: Study of encapsulation mechanism, solubility, release kinetics, and antioxidative stability [J].

Chen, Yulu ;

Su, Jiaqi ;

Dong, Wenxia ;

Xu, Duoxia ;

Cheng, Lei ;

Mao, Like ;

Gao, Yanxiang ;

Yuan, Fang .

FOOD CHEMISTRY, 2022, 383

[8] The origins of enzyme kinetics [J].

Cornish-Bowden, Athel .

FEBS LETTERS, 2013, 587 (17) :2725-2730

[9] SIMPLE GRAPHICAL METHOD FOR DETERMINING INHIBITION CONSTANTS OF MIXED, UNCOMPETITIVE AND NON-COMPETITIVE INHIBITORS [J].

CORNISHB.A .

BIOCHEMICAL JOURNAL, 1974, 137 (01) :143-144

[10] Relationships between inhibition constants, inhibitor concentrations for 50% inhibition and types of inhibition:: new ways of analysing data [J].

Cortés, A ;

Cascante, M ;

Cárdenas, ML ;

Cornish-Bowden, A .

BIOCHEMICAL JOURNAL, 2001, 357 (01) :263-268

← 1 2 3 4 →