Detection of naringin by fluorescent polarization molecularly imprinted polymer

A fluorescent magnetic surface molecular imprinting method was used to detect naringin by fluorescence polarization technology. By using SiO2-coated magnetic particles as substrate and methacrylic acid and acrylamide as monomers, a surface molecular imprinting polymer with both fluorescence and magnetic characteristics was prepared and loaded with fluorescein isothiocyanate. The binding ability of the prepared polymer was tested by fluorescence polarization and ultraviolet (UV) spectrophotometry. Compared with UV detection method, the fluorescence polarization method was more sensitive, and its limit of detection was 0.1 mg/L. The recovery of the fluorescence polarization method was higher than 81.3%. It was shown that the fluorescent magnetic surface molecular imprinting technique could be a new method to detect naringin quickly and efficiently in food.