Food peptidomic analysis of bovine milk fermented by Lacticaseibacillus casei LBC 237: In silico prediction of bioactive peptides and anticancer potential

被引:8
作者
Bellaver, Emyr Hiago
Redin, Eduarda Eliza [1 ]
da Costa, Ingrid Militao [2 ]
Moroni, Liziane Schittler [1 ]
Kempka, Aniela Pinto [1 ,3 ]
机构
[1] Santa Catarina State Univ, Dept Anim Prod & Food Sci, Multicentr Grad Program Biochem & Mol Biol, Lages, SC, Brazil
[2] Santa Catarina State Univ, Dept Food Engn & Chem Engn, Pinhalzinho, SC, Brazil
[3] Univ Estado Santa Catarina, Dept Food Engn & Chem Engn, Fernando Noronha St,BR 282,Km 573 5, BR-89870000 Pinhalzinho, SC, Brazil
关键词
Milk proteins; Fermentation; Peptide; Chemical and biochemical characteristic; Biological activity; QUORUM SENSING INHIBITORS; LACTIC-ACID BACTERIA; LACTOBACILLUS-CASEI; YOGURT; SCALE;
D O I
10.1016/j.foodres.2024.114060
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Bioactive peptides, which exhibited diverse biological activities such as anti -cancer, anti-inflammatory, bactericidal, antiviral, and quorum sensing properties, were considered promising alternative therapeutic agents. Sourced from various raw materials, particularly foods, these peptides garnered significant interest. In this context, the study focused on exploring bioactive peptides derived from bovine whole milk fermentation by Lacticaseibacillus casei LBC 237. Comprehensive peptidomic analysis and in silico predictions, with a specific emphasis on anti -cancer properties, were conducted. The study categorized peptides into BP-LBC, originating from the metabolism of L. casei LBC 237 and not matching any sequence in the Bos taurus database, and BP -MILK, matching a sequence in the Bos taurus database. Among the 143 identified peptides with potential biological activity, 33.56% were attributed to BP-LBC, while 66.43% originated from BP -MILK, demonstrating the important contribution of proteins in bovine milk in the generation of bioactive peptides. Hydrophobic peptides, enriched in Leucine, Lysine, and Proline, dominated both fractions, significantly influencing their functional properties. Pearson correlation analysis revealed inverse relationships between bioactive peptides, molecular weight, and anti -tumor activity in BP -MILK. The DGKVWEESLK peptide exhibited in silico activity against 10 different cancer cell lines. Studying the bioactive properties of peptides from familiar sources enhances the connection between food science and human health. In addition, in silico studies have been crucial in deepening our understanding of the bioactive potential of these peptides and their mode of action.
引用
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页数:15
相关论文
共 86 条
[1]   Antiviral Peptides: Identification and Validation [J].
Agarwal, Garima ;
Gabrani, Reema .
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, 2021, 27 (01) :149-168
[2]   Assessment of multifunctional activity of bioactive peptides derived from fermented milk by specific Lactobacillus plantarum strains [J].
Aguilar-Toala, J. E. ;
Santiago-Lopez, L. ;
Peres, C. M. ;
Peres, C. ;
Garcia, H. S. ;
Vallejo-Cordoba, B. ;
Gonzalez-Cordova, A. F. ;
Hernandez-Mendoza, A. .
JOURNAL OF DAIRY SCIENCE, 2017, 100 (01) :65-75
[3]   Bioactive Peptides: Synthesis, Sources, Applications, and Proposed Mechanisms of Action [J].
Akbarian, Mohsen ;
Khani, Ali ;
Eghbalpour, Sara ;
Uversky, Vladimir N. .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (03)
[4]   pdCSM-cancer: Using Graph-Based Signatures to Identify Small Molecules with Anticancer Properties [J].
Al-Jarf, Raghad ;
de Sa, Alex G. C. ;
Pires, Douglas E., V ;
Ascher, David B. .
JOURNAL OF CHEMICAL INFORMATION AND MODELING, 2021, 61 (07) :3314-3322
[5]  
Aluko R., 2012, Food Science Text Series, P37, DOI [10.1007/978-1-4614-3480-1, DOI 10.1007/978-1-4614-3480-1_3, DOI 10.1007/978-1-4614-3480-1]
[6]   Role of Probiotics in health improvement, infection control and disease treatment and management [J].
Amara, A. A. ;
Shibl, A. .
SAUDI PHARMACEUTICAL JOURNAL, 2015, 23 (02) :107-114
[7]  
[Anonymous], UniProt BLAST sequence similarity searching
[8]   Selective anticancer activity of synthetic peptides derived from the host defence peptide tritrpticin [J].
Arias, Mauricio ;
Haney, Evan F. ;
Hilchie, Ashley L. ;
Corcoran, Jennifer A. ;
Hyndman, M. Eric ;
Hancock, Robert E. W. ;
Vogel, Hans J. .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2020, 1862 (08)
[9]   Biosynthesis of the acid protease produced by Lacticaseibacillus casei LBC 237 and Limosilactobacillus fermentum LBF 433 and their potential application in the bovine milk clotting [J].
Artifon, Samantha Emanuella Sghedoni ;
Sumny, Eduarda Heck ;
Fabris, Taeline ;
Jesus, Bruna Andersen Pereira de ;
Borba Magalhaes, Maria de Lourdes ;
da Silva, Gustavo Felippe ;
Dognini, Jocinei ;
Andrade, Nathalia Coelho ;
Moroni, Liziane Schittler ;
Kempka, Aniela Pinto .
FOOD BIOSCIENCE, 2023, 54
[10]   Recent trends in the analysis of bioactive peptides in milk and dairy products [J].
Capriotti, Anna Laura ;
Cavaliere, Chiara ;
Piovesana, Susy ;
Samperi, Roberto ;
Lagana, Aldo .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2016, 408 (11) :2677-2685