Liquid biopsy for precision diagnostics and therapeutics

被引:7
作者
Anitha, Kuttiappan [1 ]
Posinasetty, Bhargavi [2 ]
Kumari, K. Naveen [3 ]
Chenchula, Santenna [4 ]
Padmavathi, R. [5 ]
Prakash, Satya [4 ]
Radhika, Chikatipalli [6 ]
机构
[1] SVKMs Narsee Monjee Inst Management Studies NMIMS, Sch Pharm & Technol Management SPTM, Dept Pharmacol, Shirpur 425405, India
[2] PROMETRIKA LLC, 100 Cambridge Pk, Cambridge, MA 02140 USA
[3] Sri Krishna Teja Pharm Coll, Tirupati 517502, Andhra Pradesh, India
[4] All India Inst Med Sci, Bhopal 462020, India
[5] SVS Med Coll, Hyderabad, Telangana, India
[6] Sri Venkateswara Coll Pharm, Chittoor, AP, India
关键词
Liquid biopsy; Oncology; Circulating tumor cells (CTCs); Cell-free nucleic acids (cfNAs); Exosomes; Circulating tumor DNA (ctDNA); Biomarkers; CIRCULATING TUMOR-CELLS; PANCREATIC-CANCER DIAGNOSIS; BREAST-CANCER; FREE DNA; NUCLEIC-ACIDS; LUNG-CANCER; BIOMARKERS; EXOSOME; METHYLATION; METASTASIS;
D O I
10.1016/j.cca.2023.117746
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Liquid biopsy (LB) has emerged as a highly promising and non-invasive diagnostic approach, particularly in the field of oncology, and has garnered interest in various medical disciplines. This technique involves the examination of biomolecules released into physiological fluids, such as urine samples, blood, and cerebrospinal fluid (CSF). The analysed biomolecules included circulating tumour DNA (ctDNA), circulating tumour cells (CTCs), cell-free DNA (cfDNA), exosomes, and other cell-free components. In contrast to conventional tissue biopsies, LB provides minimally invasive diagnostics, offering invaluable insights into tumor characteristics, treatment response, and early disease detection. This Review explores the contemporary landscape of technologies and clinical applications in the realm of LB, with a particular emphasis on the isolation and analysis of ctDNA and/or cfDNA. Various methodologies have been employed, including droplet digital polymerase chain reaction (DDP), BEAMing (beads, emulsion, amplification, and magnetics), TAm-Seq (tagged-amplicon deep sequencing), CAPPSeq (cancer personalized profiling by deep sequencing), WGBS-Seq (whole genome bisulfite sequencing), WES (whole exome sequencing), and WGS (whole-genome sequencing). Additionally, CTCs have been successfully isolated through biomarker-based cell capture, employing both positive and negative enrichment strategies based on diverse biophysical and other inherent properties. This approach also addresses challenges and limitations associated with liquid biopsy techniques, such as sensitivity, specificity, standardization and interpretability of findings. This review seeks to identify the current technologies used in liquid biopsy samples, emphasizing their significance in identifying tumor markers for cancer detection, prognosis, and treatment outcome monitoring.
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页数:12
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共 121 条
  • [71] Serial monitoring of circulating tumor DNA in patients with primary breast cancer for detection of occult metastatic disease
    Olsson, Eleonor
    Winter, Christof
    George, Anthony
    Chen, Yilun
    Howlin, Jillian
    Tang, Man-Hung Eric
    Dahlgren, Malin
    Schulz, Ralph
    Grabau, Dorthe
    van Westen, Danielle
    Ferno, Marten
    Ingvar, Christian
    Rose, Carsten
    Bendahl, Par-Ola
    Ryden, Lisa
    Borg, Ake
    Gruvberger-Saal, Sofia K.
    Jernstrom, Helena
    Saal, Lao H.
    [J]. EMBO MOLECULAR MEDICINE, 2015, 7 (08) : 1034 - 1047
  • [72] Genomic Analysis of Plasma Cell-Free DNA in Patients With Cancer
    Oxnard, Geoffrey R.
    Paweletz, Cloud P.
    Sholl, Lynette M.
    [J]. JAMA ONCOLOGY, 2017, 3 (06) : 740 - 741
  • [73] Liquid biopsy and minimal residual disease - latest advances and implications for cure
    Pantel, Klaus
    Alix-Panabieres, Catherine
    [J]. NATURE REVIEWS CLINICAL ONCOLOGY, 2019, 16 (07) : 409 - 424
  • [74] Circulating tumour cells in cancer patients: challenges and perspectives
    Pantel, Klaus
    Alix-Panabieres, Catherine
    [J]. TRENDS IN MOLECULAR MEDICINE, 2010, 16 (09) : 398 - 406
  • [75] Exploratory Analysis of TP53 Mutations in Circulating Tumour DNA as Biomarkers of Treatment Response for Patients with Relapsed High-Grade Serous Ovarian Carcinoma: A Retrospective Study
    Parkinson, Christine A.
    Gale, Davina
    Piskorz, Anna M.
    Biggs, Heather
    Hodgkin, Charlotte
    Addley, Helen
    Freeman, Sue
    Moyle, Penelope
    Sala, Evis
    Sayal, Karen
    Hosking, Karen
    Gounaris, Ioannis
    Jimenez-Linan, Mercedes
    Earl, Helena M.
    Qian, Wendi
    Rosenfeld, Nitzan
    Brenton, James D.
    [J]. PLOS MEDICINE, 2016, 13 (12)
  • [76] Considerations in the development of circulating tumor cell technology for clinical use
    Parkinson, David R.
    Dracopoli, Nicholas
    Petty, Brenda Gumbs
    Compton, Carolyn
    Cristofanilli, Massimo
    Deisseroth, Albert
    Hayes, Daniel F.
    Kapke, Gordon
    Kumar, Prasanna
    Lee, Jerry S. H.
    Liu, Minetta C.
    McCormack, Robert
    Mikulski, Stanislaw
    Nagahara, Larry
    Pantel, Klaus
    Pearson-White, Sonia
    Punnoose, Elizabeth A.
    Roadcap, Lori T.
    Schade, Andrew E.
    Scher, Howard I.
    Sigman, Caroline C.
    Kelloff, Gary J.
    [J]. JOURNAL OF TRANSLATIONAL MEDICINE, 2012, 10
  • [77] Circulating tumor cells (CTC) detection: Clinical impact and future directions
    Paterlini-Brechot, Patrizia
    Benali, Naoual Linda
    [J]. CANCER LETTERS, 2007, 253 (02) : 180 - 204
  • [78] Correlation of plasma exosomal microRNAs with the efficacy of immunotherapy in EGFR/ALK wild-type advanced non-small cell lung cancer
    Peng, Xiao-Xiao
    Yu, Ruoying
    Wu, Xue
    Wu, Shu-Yu
    Pi, Can
    Chen, Zhi-Hong
    Zhang, Xu-Chao
    Gao, Cun-Yi
    Shao, Yang W.
    Liu, Li
    Wu, Yi-Long
    Zhou, Qing
    [J]. JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2020, 8 (01)
  • [79] Emerging concepts in liquid biopsies
    Perakis, Samantha
    Speicher, Michael R.
    [J]. BMC MEDICINE, 2017, 15
  • [80] The Role of Circulating Free DNA and MicroRNA in Non-Invasive Diagnosis of HBV- and HCV-Related Hepatocellular Carcinoma
    Pezzuto, Francesca
    Buonaguro, Luigi
    Buonaguro, Franco Maria
    Tornesello, Maria Lina
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2018, 19 (04)